Transcription profiling of murine epididymal adipose tissue
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Joseph Cassady
PROVIDER: E-GEOD-1580 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice.
House Ralph L RL Cassady Joseph P JP Eisen Eugene J EJ Eling Thomas E TE Collins Jennifer B JB Grissom Sherry F SF Odle Jack J
Physiological genomics 20050501 3
Gene expression was measured during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice (n = 185) were allotted to a 2 x 2 factorial experiment consisting of either nonobese (ICR-control) or obese (M16-selected) mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or 1% t10c12-CLA. Body weight (BW) by day 14 was 12% lower in CLA- compared with LA-fed mice (P < 0.0001). By day 14, t10c12-CLA reduced weights of epididymal, mesenteric, and brown a ...[more]
Similar Datasets
Project description:Functional genomic analysis of liver tissue extracted from polygenic obese mice fed either a control (1% linoleic acid) or treatment (1% trans 10, cis 12 conjugated linoleic acid) diet after fourteen days.
2008-06-11 | E-GEOD-1594 | biostudies-arrayexpress
Project description:The trans-10, cis-12-isomer of conjugated linoleic acid (CLA) causes a rapid reduction of body and adipose mass in mice by decreasing adipocyte size and number. In addition to changes in adipose tissue, numerous studies have reported alterations in hepatic lipid metabolism. Livers of CLA fed mice gain mass due to lipid accumulation; however, the precise molecular mechanisms are unknown. To elucidate these mechanisms, we examined fatty acid composition and gene expression profiles of livers from a polygenic obese line of mice fed 1% trans-10, cis-12 CLA. Following examination of the fatty acid profile it was concluded that stearoyl CoA desaturase (SCD) activity was unaffected by CLA. Analysis of gene expression data led to the identification of 1393 genes differentially expressed in the liver of CLA fed mice at a nominal P-value of 0.01 and 775 were considered significant using a false discovery rate threshold of 0.05. Following Bonferroni correction and excluding lowly expressed transcripts, 198 genes were identified as being differentially expressed with 17 genes having ⥠2-fold change. Pathway analysis of the 775 genes considered significant using FDR found that 38 of these genes are controlled by Hepatocyte Nuclear Factor 4α, a transcription factor thought to be important in controlling liver metabolic status. The list of significant differentially expressed genes found in the liver of mice fed CLA vs. LA is linked below as a supplementary file. Experiment Overall Design: Functional genomic analysis of liver tissue extracted from polygenic obese male mice fed either a control (1% linoleic acid) or treatment (1% trans 10, cis 12 conjugated linoleic acid) diet after fourteen days. Four or five mice were pooled for each labeling. There is 1 replicate for each experiment, for a total of 4 microarrays.
2009-03-07 | E-GEOD-10865 | biostudies-arrayexpress
Project description:Functional genomic analysis of epididymal adipose tissue extracted from polygenic obese mice fed either a control (1% linoleic acid) or treatment (1% trans 10, cis 12 conjugated linoleic acid) diet after five or fourteen days. Keywords = Obesity Keywords = conjugated linoleic acid Keywords = polygenic obese mice Keywords = adiposity Keywords = lipid metabolism Keywords = apoptosis Keywords: repeat sample
2004-07-23 | GSE1580 | GEO
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2008-06-12 | E-GEOD-1501 | biostudies-arrayexpress
Project description:Functional genomic analysis of liver tissue extracted from polygenic obese mice fed either a control (1% linoleic acid) or treatment (1% trans 10, cis 12 conjugated linoleic acid) diet after fourteen days. Keywords = Obesity Keywords = conjugated linoleic acid Keywords = polygenic obese mice Keywords = lipid metabolism Keywords: repeat sample
2004-07-27 | GSE1594 | GEO
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2008-11-06 | E-GEOD-5906 | biostudies-arrayexpress
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