Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiling of human gliomas and human glioblastoma cell lines


ABSTRACT: To identify signaling pathways that are differentially regulated in human gliomas, a microarray analysis on 30 brain tumor samples (12 primary glioblastomas (GBM), 3 secondary glioblastomas (GBM-2), 8 astrocytomas (Astro) and 7 oligodendrogliomas (Oligo)) and on 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149) was performed. Normal brain tissue (NB) and normal human astrocytes (NHA) were used as a control. Kinase expression in each tumor was compared to expression in normal brain and expression values from normal human astrocytes were used as an additional control. Keywords: Kinase expression in each tumor was compared to expression in normal brain and expression values from normal human astrocytes were used as an additional control. Frozen tissue samples of human gliomas and normal brain obtained from the operating room were processed according to the guidelines of the Ethical Committee of the University Hospitals of Basel. Human brain tumor cell lines were derived from human patients. The BS149 cell line was generated at the University of Basel, Switzerland while the “LN” series were a kind gift of Erwin van Meir in Lausanne, Switzerland. Normal human astrocytes (NHAs) were purchased by Cambrex (Walkersville, MD) and cultured according to manufacturer’s recommendations.

ORGANISM(S): Homo sapiens

SUBMITTER: Michal Grzmil 

PROVIDER: E-GEOD-15824 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-β signaling pathway in human glioblastoma.

Grzmil Michal M   Morin Pier P   Lino Maria Maddalena MM   Merlo Adrian A   Frank Stephan S   Wang Yuhua Y   Moncayo Gerald G   Hemmings Brian A BA  

Cancer research 20110314 6


Glioblastoma multiforme (GBM) is the most common aggressive brain cancer with a median survival of approximately 1 year. In a search for novel molecular targets that could be therapeutically developed, our kinome-focused microarray analysis identified the MAP (mitogen-activated protein) kinase-interacting kinase 1 (MNK1) as an attractive theranostic candidate. MNK1 overexpression was confirmed in both primary GBMs and glioma cell lines. Inhibition of MNK1 activity in GBM cells by the small molec  ...[more]

Similar Datasets

2011-03-18 | GSE15824 | GEO
2014-09-26 | E-GEOD-61710 | biostudies-arrayexpress
2014-09-26 | GSE61710 | GEO
2008-04-29 | E-GEOD-9200 | biostudies-arrayexpress
2008-01-28 | E-GEOD-9385 | biostudies-arrayexpress
2018-11-20 | GSE122679 | GEO
2008-04-29 | E-GEOD-9177 | biostudies-arrayexpress
2011-03-23 | E-GEOD-22927 | biostudies-arrayexpress
2008-12-31 | E-GEOD-12305 | biostudies-arrayexpress
2021-12-30 | PXD023350 | Pride