Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse NFI-C knock out embryonic fibroblasts


ABSTRACT: The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-β signaling, extracellular matrix deposition and skin appendage pathologies. We performed a global gene expression analysis in NFI-C-/- and wild-type embryonic primary murine fibroblasts. This indicated that NFI-C acts mostly to repress gene expression in response to TGF-β1. Misregulated genes featured a prominent over-representation of regulators of connective tissue inflammation and repair. Experiment Overall Design: mRNAs were isolated from embryonic fibroblasts obtained from 3 wild-type and 3 NFI-C knock-out mice, and their levels were probed using microarrays. Prior to RNA extraction, fibroblast cultures were treated or not with TGF-β1 for 1 hour to examine the immediate response to the growth factor, or treated for 10 hours to assess the delayed response.

ORGANISM(S): Mus musculus

SUBMITTER: Milos Pjanic 

PROVIDER: E-GEOD-15871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nuclear factor I-C links platelet-derived growth factor and transforming growth factor beta1 signaling to skin wound healing progression.

Plasari Genta G   Calabrese Alessandra A   Dusserre Yves Y   Gronostajski Richard M RM   McNair Alan A   Michalik Liliane L   Mermod Nicolas N  

Molecular and cellular biology 20090914 22


Transforming growth factor beta (TGF-beta) and platelet-derived growth factor A (PDGFAlpha) play a central role in tissue morphogenesis and repair, but their interplay remain poorly understood. The nuclear factor I C (NFI-C) transcription factor has been implicated in TGF-beta signaling, extracellular matrix deposition, and skin appendage pathologies, but a potential role in skin morphogenesis or healing had not been assessed. To evaluate this possibility, we performed a global gene expression a  ...[more]

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