Unknown,Transcriptomics,Genomics,Proteomics

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Adaptation of Sulfolobus solfataricus P2 to fluctuating O2 concentrations


ABSTRACT: Sulfolobus solfataricus P2 was grown aerobically, with O2 concentrations ranging from 1.5 to 26 % (v/v; gas phase). To gain some insight in control of the respiratory system, transcriptomes of the strain cultivated in different O2 concentrations (1.5 % vs 21 %, 1.5 % vs 26 %) were compared using a DNA microarray approach. Two-condition experiments: 1.5% O2 vs two other O2 concentrations (21 or 26% O2). Biological replicates independently grown. One replicate per array. Dye swaps.

ORGANISM(S): Sulfolobus solfataricus

SUBMITTER: Richard Auria 

PROVIDER: E-GEOD-16043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effect of O2 concentrations on Sulfolobus solfataricus P2.

Simon Gwénola G   Walther Jasper J   Zabeti Nathalie N   Combet-Blanc Yannick Y   Auria Richard R   van der Oost John J   Casalot Laurence L  

FEMS microbiology letters 20090818 2


Sulfolobus solfataricus P2 was grown aerobically at various O(2) concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O(2) (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O(2), the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O(2). For 16-24% O(2), standard growth was observed. For 1.5-15% O(2), growth was comparable with the reference,  ...[more]

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