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The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans

ABSTRACT: We generated animals carrying a genomically integrated mir-124 promoter::gfp transgene and identified mir-124 promoter::GFP labelled cells as a subset of the C. elegans sensory neurons. We used fluorescence activated cell sorting (FACS) to isolate four distinct cell populations: mir-124 expressing (GFP+) and non-expressing (GFP-) cells from both wild-type and mutant animals. RNA samples obtained from the four cell populations were used for Affymetrix gene expression analysis to study the effect of mir-124 deletion on the transcriptome of mir-124 expressing (GFP+) and non-expressing (GFP-) cells. Affymetrix gene expression data from isolated populations of mir-124 expressing (GFP+) and non-expressing (GFP-) cells obtained from wild-type and mir-124 mutant C. elegans (in biological triplicates)

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Shai Shaham

PROVIDER: E-GEOD-16050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans.

Clark Alejandra M AM Goldstein Leonard D LD Tevlin Maya M Tavaré Simon S Shaham Shai S Miska Eric A EA

Nucleic acids research 20100221 11

miR-124 is a highly conserved microRNA (miRNA) whose in vivo function is poorly understood. Here, we identify miR-124 targets based on the analysis of the first mir-124 mutant in any organism. We find that miR-124 is expressed in many sensory neurons in Caenorhabditis elegans and onset of expression coincides with neuronal morphogenesis. We analyzed the transcriptome of miR-124 expressing and nonexpressing cells from wild-type and mir-124 mutants. We observe that many targets are co-expressed wi ...[more]

PMID: 20176573

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The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans

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