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SNEP: Simultaneous detection of nucleotide and expression polymorphisms using Affymetrix GeneChip


ABSTRACT: Nucleotide polymorphisms can potentially influence the hybridization of mRNA to 25-mer oligonucleotides. Because Affymetrix Rice Genome Array was designated mainly for Nipponbare genome of Oryza sativa, the expression level of other varieties could not be estimated correctly. We tried to apply new approaches to estimate expression level by discerning the probe-level differential hybridization. Nipponbare and 93-11 replicates were analyzed.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Nori Kurata 

PROVIDER: E-GEOD-16265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SNEP: Simultaneous detection of nucleotide and expression polymorphisms using Affymetrix GeneChip.

Fujisawa Hironori H   Horiuchi Youko Y   Harushima Yoshiaki Y   Takada Toyoyuki T   Eguchi Shinto S   Mochizuki Takako T   Sakaguchi Takayuki T   Shiroishi Toshihiko T   Kurata Nori N  

BMC bioinformatics 20090506


<h4>Background</h4>High-density short oligonucleotide microarrays are useful tools for studying biodiversity, because they can be used to investigate both nucleotide and expression polymorphisms. However, when different strains (or species) produce different signal intensities after mRNA hybridization, it is not easy to determine whether the signal intensities were affected by nucleotide or expression polymorphisms. To overcome this difficulty, nucleotide and expression polymorphisms are current  ...[more]

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