Gene expression data from gastrocnemius muscle (m.Gas) in young adult mice
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ABSTRACT: This study examined the effects of castration and testosterone replacement on global differential gene transcription in the gastrocnemius muscle (m.Gas) in young adult mice over 14-days. Each of the 3 treatment groups was composed of 3 independent biological replicates, totaling 9 arrays. Equal amounts of the highest quality total RNA (4 µg) from three animals were pooled for each biological replicate, totaling the use of 27 animals in the 9-array experiment. The balanced design of this study enabled the use of a 1-way ANOVA, which was used to statistically determine which of the >34,000 genes (>45,000 probes) were differentially expressed between groups. The three pair-wise comparisons were: Castration vs. Sham (castration (C) effect); Testosterone vs. Castration (testosterone replacement (T) effect); and Testosterone vs. Sham (overall effects).
ORGANISM(S): Mus musculus
SUBMITTER: Akbar Siddiqui
PROVIDER: E-GEOD-16486 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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