Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide map of PCL2 (Polycomb-like 2) enrichment in undifferentiated mouse embryonic stem cells (ESCs)


ABSTRACT: In total 8,736,191 reads from IP fraction and 9,958,984 reads from Wash fraction uniquely mapped to the mouse genome (mm9). Settings for enrichment analysis: maxSpacing=500; minHits=20; minPeakHits=6; minRatio=3. 4,701 regions have been identified as enriched in the IP fraction. In order to get a rough idea of false discovery rate (FDR), enrichment analysis using Wash fraction as test and IP fraction as control was performed, 15 regions was found. This suggests that the FDR likely is around 0.3%. Keywords: Epigenetics Examination of PCL2 enrichment in mouse ESCs.

ORGANISM(S): Mus musculus

SUBMITTER: William Stanford 

PROVIDER: E-GEOD-16526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation.

Walker Emily E   Chang Wing Y WY   Hunkapiller Julie J   Cagney Gerard G   Garcha Kamal K   Torchia Joseph J   Krogan Nevan J NJ   Reiter Jeremy F JF   Stanford William L WL  

Cell stem cell 20100201 2


Polycomb group (PcG) proteins are conserved epigenetic transcriptional repressors that control numerous developmental gene expression programs and have recently been implicated in modulating embryonic stem cell (ESC) fate. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC-fate determination. Using multiple biochemical strategies, we provide evidence that PCL2  ...[more]

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