Unknown,Transcriptomics,Genomics,Proteomics

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Differential transcriptomic responses of Biomphalaria glabrata to bacteria and metazoan parasites


ABSTRACT: Differential transcriptomic responses of Biomphalaria glabrata (Gastropoda, Mollusca) to bacteria and metazoan parasites Schistosoma mansoni and Echinostoma paraensei (Digenea, Platyhelminthes). Transcriptional profiling of Biomphalaria glabrata comparing control uninfected M-line B. glabrata with five experimental groups. The experimental groups are: wounded but not infected M-line, Escherichia coli infected, Micrococcus luteus infected, Echinostoma paraensei infected and Schistosoma mansoni infected at 12 hours time point post infection. Seven groups of samples: two controls, wounded, two bacterial- and two trematode-infected B. glabrata were analyzed in triplicate, using universal RNA reference.

ORGANISM(S): Biomphalaria glabrata

SUBMITTER: Eric Loker 

PROVIDER: E-GEOD-16596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential transcriptomic responses of Biomphalaria glabrata (Gastropoda, Mollusca) to bacteria and metazoan parasites, Schistosoma mansoni and Echinostoma paraensei (Digenea, Platyhelminthes).

Adema Coen M CM   Hanington Patrick C PC   Lun Cheng-Man CM   Rosenberg George H GH   Aragon Anthony D AD   Stout Barbara A BA   Lennard Richard Mara L ML   Gross Paul S PS   Loker Eric S ES  

Molecular immunology 20091203 4


A 70-mer-oligonucleotide-based microarray (1152 features) that emphasizes stress and immune responses factors was constructed to study transcriptomic responses of the snail Biomphalaria glabrata to different immune challenges. In addition to sequences with relevant putative ID and Gene Ontology (GO) annotation, the array features non-immune factors and unknown B. glabrata ESTs for functional gene discovery. The transcription profiles of B. glabrata (3 biological replicates, each a pool of 5 snai  ...[more]

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