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MiRNAs in activated mouse CD4 T cells by Bcl-6 overexpression

ABSTRACT: Compare miRNA expression profiles in mouse CD4 T cells overexpression of Bcl-6 vs empty vector Mouse naÃ¯ve CD4+ T cells were transduced with a Bcl-6-expressing retrovirus or the control vector and cultured in Th0 condition for 2 days. Cells expressing medium to high levels of GFP were sorted and total RNAs were isolated using mirVana miRNA Isolation Kit (Applied Biosystems). MiRNA expression was profiled using Agilent 8x15K mouse miRNA microarray miRNA-v1_95_May07 (miRBase Release 9.2) by Ramaciotti Centre for Gene Function Analysis (Sydney). Each sample was run in duplicate.

ORGANISM(S): Mus musculus

SUBMITTER: Louis Tsai

PROVIDER: E-GEOD-17000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcriptional repressor Bcl-6 directs T follicular helper cell lineage commitment.

Yu Di D Rao Sudha S Tsai Louis M LM Lee Sau K SK He Yiqing Y Sutcliffe Elissa L EL Srivastava Monika M Linterman Michelle M Zheng Lei L Simpson Nicholas N Ellyard Julia I JI Parish Ian A IA Ma Cindy S CS Li Qi-Jing QJ Parish Christopher R CR Mackay Charles R CR Vinuesa Carola G CG

Immunity 20090723 3

Follicular helper T (Tfh) cells provide selection signals to germinal center B cells, which is essential for long-lived antibody responses. High CXCR5 and low CCR7 expression facilitates their homing to B cell follicles and distinguishes them from T helper 1 (Th1), Th2, and Th17 cells. Here, we showed that Bcl-6 directs Tfh cell differentiation: Bcl-6-deficient T cells failed to develop into Tfh cells and could not sustain germinal center responses, whereas forced expression of Bcl-6 in CD4(+) T ...[more]

PMID: 19631565

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miRNAs in activated mouse CD4 T cells by Bcl-6 overexpression

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