Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of mouse embryonic fibroblasts Cre infected vs. control Vector infected

ABSTRACT: To examine the transcriptional changes that occur following alpha4 deletion, RNA microarray analysis was performed using RNA from alpha4-floxed MEFs 48 hours after infection with the Cre-containing or Vector control virus.

ORGANISM(S): Mus musculus

SUBMITTER: Mei Kong

PROVIDER: E-GEOD-1708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The PP2A-associated protein alpha4 is an essential inhibitor of apoptosis.

Kong Mei M Fox Casey J CJ Mu James J Solt Laura L Xu Anne A Cinalli Ryan M RM Birnbaum Morris J MJ Lindsten Tullia T Thompson Craig B CB

Science (New York, N.Y.) 20041001 5696

Despite evidence that protein kinases are regulators of apoptosis, a specific role for phosphatases in regulating cell survival has not been established. Here we show that alpha4, a noncatalytic subunit of protein phosphatase 2A (PP2A), is required to repress apoptosis in murine cells. alpha4 is a nonredundant regulator of the dephosphorylation of the transcription factors c-Jun and p53. As a result of alpha4 deletion, multiple proapoptotic genes were transcribed. Either inhibition of new protei ...[more]

PMID: 15499020

Similar Datasets

Comparison of transcriptional changes in Cre infected with Vector infected alpha4-floxed MEFs

Project description:To examine the transcriptional changes that occur following alpha4 deletion, RNA microarray analysis was performed using RNA from alpha4-floxed MEFs 48 hours after infection with the Cre-containing or Vector control virus. Keywords: other

2004-09-01 | GSE1708 | GEO

Cre-ERT mediated antiviral induction

2016-05-18 | GSE72024 | GEO

Cre-ERT mediated antiviral induction

Project description:In this work, we aimed at studying the impact of microRNAs on antiviral responses, using inducible deletion of one or two copies of Dicer1 (in Dicer-floxed x EsrCRE+ MEFs). Unexpectedly, we discovered that Cre-ERT activation by hydroxytamoxifen (OHT) alone, independent of Dicer1 deletion, had a strong impact on antiviral responses. These microarrays were performed on SV40 immortalized Dicer1 fl/fl EsrCRE+ MEFs, Dicer1 fl/wt EsrCRE+ MEFs, 96 h after initial OHT treatment, and SV40 immortalized Dicer1 wt/wt EsrCRE+ MEFs, Dicer1 wt/wt EsrCRE-neg MEFs (Wild Type MEFs), 48 h after initial OHT treatment. These analyses revealed the strong induction of several antiviral genes (Rsad2, Cxcl10...), following Cre-ERT activation. SV40 immortalized mouse embryonic fibroblasts from various genotypes were stimulated 24h with 500nM hydroxytamoxifen, and further expanded for 24 or 72h. Each sample was analyzed on Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray Each sample is in simplicate - no biological replicates; sample 1-4 were processed/analyzed on a different microarray slide to 5-8 (different day/operator)

2016-05-18 | E-GEOD-72024 | biostudies-arrayexpress

Mus musculus

Project description:Comparison of transcriptional changes in Cre infected with Vector infected alpha4-floxed MEFs

| PRJNA90633 | ENA

RNAseq comparing mouse Cre-loxP-induced EWSR1-ATF1 expression from Rosa26 versus Ewsr1-Atf1 translocation

Project description:Mice formed tumors either after a TATCre-induced floxed-STOP removal from an EWSR1-ATF1 human cDNA expression vector targeted to the Rosa26 endogenous pseudogene or after an Hprt-Cre-initiated translocation between Ewsr1 and Atf1 genes on chromosomes 11 and 15. From total RNA, libraries were generated using the RiboZero method and sequenced on an Ilumina NovaSeq instrument to permit comparison to each other.

2020-09-30 | GSE158725 | GEO

B cells sorted from VHL floxed MB1- Cre

Project description:B cells sorted from VHL floxed MB1- Cre

| PRJNA531628 | ENA

Gene expression in IRfl/fl Cre- and IRfl/fl Cre+ mice

Project description:The floxed insulin receptor was specifically targetted for deletion in the mammary gland using a mouse strain bearing Cre recombinase under the BLG promoter. Data analysis: Genespring was used for data analysis All entities (28853) were filtered on variance between samples, eliminating from further analysis of genes whose coefficient of variation was greater than 50%. The remaining 2239 genes were filtered on expression levels between 20 and 20,000 intensity units An unpaired T-test was used to find genes that differed significantly (P<0.05) between experimentals and controls The remaining 2014 genes were filtered for a 1.1 absolute fold change between experimentals and controls. Of the remaining expression of 890 genes was down in the Cre+ mice and expression was up for 457 genes in the Cre+ mice. Mouse strains containing a floxed insulin receptor (IRfl/fl) were crossed with a mouse expressing Cre recombinase from a BLG promoter RNA was applied to Affymetrix MoGene_1_0-st-v1 chips Three replicates from each strain were used for analysis.

2013-06-18 | E-GEOD-48009 | biostudies-arrayexpress

P0-cre marked neural crest cells in murine embryo

Project description:To elucidate the molecular features of craniofacial versus trunk neural crest cells (NCCs), we utilized P0-Cre/Floxed-EGFP mice that specifically label NCCs (Yamauchi et al., 1999 (PMID 10419695)). Craniofacial and trunk regions were isolated from P0-Cre/Floxed-EGFP mouse embryos at embryonic day E12.5, and dissociated cells were analyzed by flow cytometory in regard to the intensity of EGFP.

2014-08-01 | GSE39191 | GEO

RNA sequencing of Mpc1 floxed mouse prostate organoids transduced with RFP control or Cre virus and treated -/+ small molecule MPC inhibitor UK5099

Project description:The goal of this study was to determine how knockout or pharmacological inhibition of the mitochondrial pyruvate carrier (MPC) alters lineage identity in the prostate. We isolated basal cells from Mpc1 floxed mouse prostates and transduced with a control RFP virus or Cre recombinase virus. We then plated into organoid culture and treated with either vehicle or the small molecule MPC inhibitor UK5099 for one week.

2023-09-01 | GSE221022 | GEO

Mouse Embryo Sox1 cre-dependent knockout of EED

Project description:Floxed EED homozygote mice are knocked-out by Sox1-driven Cre to remove H3K27me3 during neurogenesis

2018-03-01 | GSE111232 | GEO