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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse embryonic fibroblasts Cre infected vs. control Vector infected

ABSTRACT: To examine the transcriptional changes that occur following alpha4 deletion, RNA microarray analysis was performed using RNA from alpha4-floxed MEFs 48 hours after infection with the Cre-containing or Vector control virus.

ORGANISM(S): Mus musculus

SUBMITTER: Mei Kong

PROVIDER: E-GEOD-1708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The PP2A-associated protein alpha4 is an essential inhibitor of apoptosis.

Kong Mei M Fox Casey J CJ Mu James J Solt Laura L Xu Anne A Cinalli Ryan M RM Birnbaum Morris J MJ Lindsten Tullia T Thompson Craig B CB

Science (New York, N.Y.) 20041001 5696

Despite evidence that protein kinases are regulators of apoptosis, a specific role for phosphatases in regulating cell survival has not been established. Here we show that alpha4, a noncatalytic subunit of protein phosphatase 2A (PP2A), is required to repress apoptosis in murine cells. alpha4 is a nonredundant regulator of the dephosphorylation of the transcription factors c-Jun and p53. As a result of alpha4 deletion, multiple proapoptotic genes were transcribed. Either inhibition of new protei ...[more]

PMID: 15499020

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Comparison of transcriptional changes in Cre infected with Vector infected alpha4-floxed MEFs

Project description:To examine the transcriptional changes that occur following alpha4 deletion, RNA microarray analysis was performed using RNA from alpha4-floxed MEFs 48 hours after infection with the Cre-containing or Vector control virus. Keywords: other

2004-09-01 | GSE1708 | GEO

Cre-ERT mediated antiviral induction

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Cre-ERT mediated antiviral induction

Project description:In this work, we aimed at studying the impact of microRNAs on antiviral responses, using inducible deletion of one or two copies of Dicer1 (in Dicer-floxed x EsrCRE+ MEFs). Unexpectedly, we discovered that Cre-ERT activation by hydroxytamoxifen (OHT) alone, independent of Dicer1 deletion, had a strong impact on antiviral responses. These microarrays were performed on SV40 immortalized Dicer1 fl/fl EsrCRE+ MEFs, Dicer1 fl/wt EsrCRE+ MEFs, 96 h after initial OHT treatment, and SV40 immortalized Dicer1 wt/wt EsrCRE+ MEFs, Dicer1 wt/wt EsrCRE-neg MEFs (Wild Type MEFs), 48 h after initial OHT treatment. These analyses revealed the strong induction of several antiviral genes (Rsad2, Cxcl10...), following Cre-ERT activation. SV40 immortalized mouse embryonic fibroblasts from various genotypes were stimulated 24h with 500nM hydroxytamoxifen, and further expanded for 24 or 72h. Each sample was analyzed on Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray Each sample is in simplicate - no biological replicates; sample 1-4 were processed/analyzed on a different microarray slide to 5-8 (different day/operator)

2016-05-18 | E-GEOD-72024 | biostudies-arrayexpress

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Project description:Comparison of transcriptional changes in Cre infected with Vector infected alpha4-floxed MEFs

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B cells sorted from VHL floxed MB1- Cre

Project description:B cells sorted from VHL floxed MB1- Cre

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RNAseq comparing mouse Cre-loxP-induced EWSR1-ATF1 expression from Rosa26 versus Ewsr1-Atf1 translocation

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Gene expression in IRfl/fl Cre- and IRfl/fl Cre+ mice

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RNA sequencing of Mpc1 floxed mouse prostate organoids transduced with RFP control or Cre virus and treated -/+ small molecule MPC inhibitor UK5099

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