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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation

ABSTRACT: To measure on a global scale the SV40 large T antigen-mediated changes in gene transcription in mouse embryo fibroblasts differing in the expression of p53. Samples of total RNA from 3T3 (wt p53) and 10-1 (p53-null) mouse embryo fibroblasts stably transfected with SV40 large T-antigen expressing pMYcLT vector (3T3 LT and 10-1 LT cells) or empty pMYsIG vector (3T3 IG and 10-1 IG cells) were subjected to gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Genrich Tolstonog

PROVIDER: E-GEOD-17178 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation.

Hermannstädter Andrea A Ziegler Christine C Kühl Marion M Deppert Wolfgang W Tolstonog Genrich V GV

Journal of virology 20090722 19

Abortive infection of BALB/c mouse embryo fibroblasts differing in p53 gene status (p53(+/+) versus p53(-/)(-)) with simian virus 40 (SV40) revealed a quantitatively and qualitatively decreased transformation efficiency in p53(-/-) cells compared to p53(+/+) cells, suggesting a supportive effect of wild-type (wt) p53 in the SV40 transformation process. SV40 transformation efficiency also was low in immortalized p53(-/-) BALB/c 10-1 cells but could be restored to approximately the level in immort ...[more]

PMID: 19625393

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Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens

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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation

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GSEp53_v_Con

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GSEp53_v_Con

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