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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation

ABSTRACT: To measure on a global scale the SV40 large T antigen-mediated changes in gene transcription in mouse embryo fibroblasts differing in the expression of p53. Samples of total RNA from 3T3 (wt p53) and 10-1 (p53-null) mouse embryo fibroblasts stably transfected with SV40 large T-antigen expressing pMYcLT vector (3T3 LT and 10-1 LT cells) or empty pMYsIG vector (3T3 IG and 10-1 IG cells) were subjected to gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Genrich Tolstonog

PROVIDER: E-GEOD-17178 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation.

Hermannstädter Andrea A Ziegler Christine C Kühl Marion M Deppert Wolfgang W Tolstonog Genrich V GV

Journal of virology 20090722 19

Abortive infection of BALB/c mouse embryo fibroblasts differing in p53 gene status (p53(+/+) versus p53(-/)(-)) with simian virus 40 (SV40) revealed a quantitatively and qualitatively decreased transformation efficiency in p53(-/-) cells compared to p53(+/+) cells, suggesting a supportive effect of wild-type (wt) p53 in the SV40 transformation process. SV40 transformation efficiency also was low in immortalized p53(-/-) BALB/c 10-1 cells but could be restored to approximately the level in immort ...[more]

PMID: 19625393

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Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens

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Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation

Project description:To measure on a global scale the SV40 large T antigen-mediated changes in gene transcription in mouse embryo fibroblasts differing in the expression of p53.

2009-07-18 | GSE17178 | GEO

GSEp53_v_Con

Project description:HMF3A cells, created from adult human mammary fibroblasts by immortalisation with a thermolabile SV40 large T antigen and the catalytic sub-unit of human telomerase, undergo co-ordinated induction of cellular senescence upon inactivation of T antigen. Given the importance of p53, we sought to determine what gene changes were affected if its activation was inhibited. To do this we used the Genetic Suppressor Element (GSE)56 which inhibits the transcriptional activity of p53 (Ossovskaya, 1996). The presence of the GSEp53 delayed the arrest of the HMF3A cells when LT was inactivated. However the cells did arrest. We thus examined the array profiles for 3A-GSEp53 cells after 2 and 7 days of LT inactivation (against 3A-GSEp53 cells at 33Â°C) and compared these to the profiles for 3A-puro cells. Keywords = p53 Keywords = LT antigen Keywords = fibroblast Keywords = senescence Keywords = Rb

2010-06-09 | E-GEOD-689 | biostudies-arrayexpress

GSEp53_v_Con

Project description:HMF3A cells, created from adult human mammary fibroblasts by immortalisation with a thermolabile SV40 large T antigen and the catalytic sub-unit of human telomerase, undergo co-ordinated induction of cellular senescence upon inactivation of T antigen. Given the importance of p53, we sought to determine what gene changes were affected if its activation was inhibited. To do this we used the Genetic Suppressor Element (GSE)56 which inhibits the transcriptional activity of p53 (Ossovskaya, 1996). The presence of the GSEp53 delayed the arrest of the HMF3A cells when LT was inactivated. However the cells did arrest. We thus examined the array profiles for 3A-GSEp53 cells after 2 and 7 days of LT inactivation (against 3A-GSEp53 cells at 33°C) and compared these to the profiles for 3A-puro cells. Keywords = p53 Keywords = LT antigen Keywords = fibroblast Keywords = senescence Keywords = Rb Keywords: other

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2022-02-16 | PXD021069 | Pride

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2012-10-26 | GSE40567 | GEO

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Global analysis of gene expression in REF52 cells expressing SV40 T antigens

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