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Transcription profiling of human B cell subtypes derived from peripheral blood or umbilical cord blood.

ABSTRACT: Goals/objectives: to identify various gene expression in B cell subsets derived from human PBMC and cord blood Sample: human PBMC and human cord blood Cell subsets: memory B cell (CD20+CD10-CD27+), naive B cell (CD20+CD10-CD27-), CD21lo transitional B cell (CD20+CD10+CD27-CD21lo), CD21hi transitional B cell (CD20+CD10+CD27-CD21hi) Number of sample: PBMC (pooled from 2 donors), cord blood (pooled from 2-3 donors) Number of repeats: 2 Microarray: Affymetrix HU133Plus2 RNA extraction: Trizol (Invitrogen) RNA preparation/hybridization: All according to affymetrix protocol

ORGANISM(S): Homo sapiens

SUBMITTER: Santi Suryani

PROVIDER: E-GEOD-17186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B cells.

Suryani Santi S Fulcher David A DA Santner-Nanan Brigitte B Nanan Ralph R Wong Melanie M Shaw Peter J PJ Gibson John J Williams Andrew A Tangye Stuart G SG

Blood 20091117 3

The transitional stage of B-cell development represents an important step where autoreactive cells are deleted, allowing the generation of a mature functional B-cell repertoire. In mice, 3 subsets of transitional B cells have been identified. In contrast, most studies of human transitional B cells have focused on a single subset defined as CD24(hi)CD38(hi) B cells. Here, we have identified 2 subsets of human transitional B cells based on the differential expression of CD21. CD21(hi) transitional ...[more]

PMID: 19965666

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