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Transcriptional profile comparison of M. tuberculosis PDIM spontaneous mutants

ABSTRACT: Transcriptional profile comparison among PDIM positive and negative isolates. Standard in vitro exponential phase cultures of M. tuberculosis H37Rv ATCC27294 were plated on 7H11 plates. Individual colonies were sub-cultured in 7H9 broth and metabolically labeled with [14C] propionic acid. The apolar lipid fraction was then extracted from those cultures and assayed for PDIM (phthiocerol dimycocerosate) content via TLC. Individual isolates producing and deficient in PDIM were selected for performing transcriptional profile comparisons. Four M. tuberculosis clones derived from the same parental strain were compared. Two of them were PDIM producers while the other two were PDIM deficient. Two or four replicates were performed for each comparison using two different biological samples.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Pilar Domenech 

PROVIDER: E-GEOD-17234 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rapid and spontaneous loss of phthiocerol dimycocerosate (PDIM) from Mycobacterium tuberculosis grown in vitro: implications for virulence studies.

Domenech Pilar P   Reed Michael B MB  

Microbiology (Reading, England) 20090806 Pt 11

Isolated in vitro more than half a century ago, the H37Rv strain of Mycobacterium tuberculosis still remains the strain of choice for the majority of laboratories conducting in vivo studies of TB pathogenesis. In this report we reveal that H37Rv is highly prone to losing the ability to synthesize the cell wall lipid phthiocerol dimycocerosate (PDIM) during extended periods of in vitro culture. In addition, H37Rv stocks that have been held in vitro for even a short length of time should be though  ...[more]

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