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Expression profile of splenocytes with glucose-6-phosphate isomerase (GPI) induced arthritis

ABSTRACT: To explore TNF-related genes in GPI-induced arthritis, we performed GeneChip analysis using arthritic splenocytes and control-immunized splenocytes. Among the arrayed TNFalpha-related genes, TIARP mRNA was highly expressed in arthritic splenocytes, with levels exceeding more than 20-times the control splenocytes The spleens of three GPI-GST (MW=89 kD) (300 Âµg) -immunized DBA/1 mice were harvested on day 10. As a control, the spleens of three GST (MW=26 kd) (100 Âµg) -immunized DBA/1 mice were used. Total RNA was extracted from the splenocytes using Isogen (Nippon gene), then 15 ug of RNA was utilized for cDNA synthesis by reverse transcription followed by synthesis of biotinylated cRNA through in vitro transcription. After cRNA fragmentation, hybridization with mouse 430A2.0 GeneChip (Affymetrix, Santa Clara, CA) with probes for 43,000 mouse genes ESTs was performed according to the protocol provided by the manufacturer. Analysis was performed by gene expression software

ORGANISM(S): Mus musculus

SUBMITTER: Isao Matsumoto

PROVIDER: E-GEOD-17272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profile of splenocytes with glucose-6-phosphate isomerase (GPI) induced arthritis

Project description:To explore TNF-related genes in GPI-induced arthritis, we performed GeneChip analysis using arthritic splenocytes and control-immunized splenocytes. Among the arrayed TNFalpha-related genes, TIARP mRNA was highly expressed in arthritic splenocytes, with levels exceeding more than 20-times the control splenocytes

2009-07-24 | GSE17272 | GEO

Expression data from murine natural killer cell subsets based on Ly49 receptor expression in C57BL/6 mice (C/I+ versus Ly49G2+ NK cells)

Project description:Natural killer (NK) cells have differential expression of inhibitory and activating receptors. NK cells that express inhibitory receptors with high binding affinity to self are considered licensed and normally functional cells, while those with low affinity are unlicensed are considered hyporesponsive. Microarray analysis was performed to better understand gene expression differences between these two subpopulations of NK cells after culturing the NK cells with IL-2 from splenocytes isolated from C57BL/6 mice and then sorting for CD3-CD122+Ly49G2+Ly49C/I- or CD3-CD122+Ly49G2-Ly49C/I+. Gene fold expression differences were compared between the Ly49G2+ and Ly49C/I+ NK cells to better understand the functional roles and characterisitics of these cells. A total of 6 samples were analyzed, 3 for the CD3-CD122+Ly49G2+Ly49C/I- NK cells and 3 for the CD3-CD122+Ly49G2-Ly49C/I+. Samples were obtained from C57BL/5 splenocytes that were T cells depleted through anti-Thy1.2 and complement mediated depletion and cultured for 7 days with IL-2. Fold changes greater than 1.5 were recorded.

2014-09-01 | E-GEOD-56662 | biostudies-arrayexpress

Fibrinogen-reactive T cells transfer disease to naÃ¯ve mice

Project description:Splenocytes harvested from mice with FIA were cultured in vitro and stimulated with 0.01 mg/ml fibrinogen for 3 days. Enriched T cells were transferred into 6-week-old naÃ¯ve SJL mice, which developed visible signs of arthritis within 2 weeks. Synovial array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B cell responses against peptides representing native fibrinogen, and further spreading of the responses to target collagen type V, cartilage gp39, and citrullinated vimentin. Custom-spotted protein slides were with plasma samples from individual mice. Four slides were probed with plasma derived from naÃ¯ve mice and five slides were probed with plasma derived from mice injected with FIA T cells.

2010-05-05 | E-GEOD-18025 | biostudies-arrayexpress

Heterogeneity in allospecific T cell function in transplant tolerant hosts determines susceptibility to rejection following infection

Project description:CD4+CD45.1+ TCR75/Rag-/- T cells (TCR75) were adoptively transferred into CD45.2+ B6 hosts either untransplanted and immunized with B/c donor splenocytes i.p. (UnTx+DST) to induce memory, or prior to transplantation of a B/c heart (HTx) in the presence of anti-CD154 (day 0, day 7, day 14) and DST (day 0) to induce tolerance (Tol). A group of tolerant mice were infected with Lm on d30 post-transplantation (Tol+Lm). CD45.1+ TCR75 cells were harvested from the spleen and lymph nodes on d35-37 post-transplantation in all groups, enumerated and subjected to bulk RNAseq.

2023-09-29 | E-MTAB-13333 | biostudies-arrayexpress

Treadmill physical exercise on postarthritic phase of collagen induced arthritis in DBA1 mice_transcriptome responses on tarsal joint

Project description:Gene expression profiles in the tarsal joints of DBA/1 mice whit collagen-induced arthritis that performed treadmill exercise in the post-arthritic phase vs. those who were not exercised were compared. Exercise induced the upregulation of inflammatory genes and signaling pathways.

2022-08-30 | GSE212263 | GEO

Treadmill physical exercise on prearthritic phase of collagen induced arthritis in DBA1 mice_transcriptome responses on tarsal joint

Project description:Gene expression profiles in the tarsal joints of DBA/1 mice whit collagen-induced arthritis that performed treadmill exercise in the pre-arthritic phase vs. those who were not exercised were compared. Exercise induced the downregulation of inflammatroy genes and signaling pathways.

2022-08-30 | GSE212262 | GEO

Mus musculus

Project description:profiling of hypothalamus from arthritic DBA/1 mice vs. control DBA/1 mice

| PRJNA105037 | ENA

Mus musculus

Project description:profiling of midbrain from arthritic DBA/1 mice vs. control DBA/1 mice

| PRJNA105041 | ENA

Mus musculus

Project description:profiling of prefrontal cortex from arthritic DBA/1 mice vs. control DBA/1 mice

| PRJNA105043 | ENA

Transcription profiling of mouse splenic CD4+ cells after surgical induction of sepsis vs. controls reveals a distinct transcriptional response 6 hours after sepsis induction

Project description:Lymphocytes are adversely affected during sepsis. Some CD4+ splenocytes undergo apoptosis while others become Th2 polarized. The molecular determinants of these phenotypic changes are not known. Here we compare the transcriptional response of septic CD4 splenocytes to CD4 splenocytes from sham-manipulated animals 6h after sepsis and identify an early transcriptional component to the septic CD4+ splenocyte phenotype. CD4+ splenocytes were isolated 6h after the surgical induction of sepsis for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain a homogeneous cell population in order to reduce any effects of cellular heterogeneity on expression profiles. To that end, immunomagnetic negative selection was used to enrich CD4+ splenocyte populations to ~91%. (n=5 biological replicates each CLP and sham)

2007-12-07 | E-GEOD-4479 | biostudies-arrayexpress

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