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Transcription profiling of bone marrow samples from human acute myeloid leukemia patients

ABSTRACT: Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The labeled RNA was then fragmented and hybridazed to HU-133A oligonucleotide array (Affymetrix, Santa Clara, CA), which contained 22,283 probe sets, according to Affymetrix protocols. The arrays were scanned using Gene Array Scanner (Affymetrix).

ORGANISM(S): Homo sapiens

DISEASE(S): acute erythroid leukemia

SUBMITTER: Norma Carmen Gutierrez

PROVIDER: E-GEOD-1729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profile reveals deregulation of genes with relevant functions in the different subclasses of acute myeloid leukemia.

Gutiérrez N C NC López-Pérez R R Hernández J M JM Isidro I I González B B Delgado M M Fermiñán E E García J L JL Vázquez L L González M M San Miguel J F JF

Leukemia 20050301 3

Bone marrow samples from 43 adult patients with de novo diagnosed acute myeloid leukemia (AML)--10 acute promyelocytic leukemias (APL) with t(15;17), four AML with inv(16), seven monocytic leukemias and 22 nonmonocytic leukemias--were analyzed using high-density oligonucleotide microarrays. Hierarchical clustering analysis segregated APL, AML with inv(16), monocytic leukemias and the remaining AML into separate groups. A set of only 21 genes was able to assign AML to one of these three classes: ...[more]

PMID: 15674361

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Gene expression profile of acute myeloid leukemia

Project description:Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The labeled RNA was then fragmented and hybridazed to HU-133A oligonucleotide array (Affymetrix, Santa Clara, CA), which contained 22,283 probe sets, according to Affymetrix protocols. The arrays were scanned using Gene Array Scanner (Affymetrix). Keywords = expression profile Keywords = myeloid leukemia Keywords = microarrays Keywords: other

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Comparison of transcripts from the anterior tibialis, gastrocnemius, and liver of glycogen synthase WT, GSL30 or KO mice

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