Unknown,Transcriptomics,Genomics,Proteomics

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Overexpression of USF1 in HEK293T cells


ABSTRACT: Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher Plaisier 

PROVIDER: E-GEOD-17300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A systems genetics approach implicates USF1, FADS3, and other causal candidate genes for familial combined hyperlipidemia.

Plaisier Christopher L CL   Horvath Steve S   Huertas-Vazquez Adriana A   Cruz-Bautista Ivette I   Herrera Miguel F MF   Tusie-Luna Teresa T   Aguilar-Salinas Carlos C   Pajukanta Päivi P  

PLoS genetics 20090911 9


We hypothesized that a common SNP in the 3' untranslated region of the upstream transcription factor 1 (USF1), rs3737787, may affect lipid traits by influencing gene expression levels, and we investigated this possibility utilizing the Mexican population, which has a high predisposition to dyslipidemia. We first associated rs3737787 genotypes in Mexican Familial Combined Hyperlipidemia (FCHL) case/control fat biopsies, with global expression patterns. To identify sets of co-expressed genes co-re  ...[more]

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