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Transcriptional effects of the IWR1 deletion

ABSTRACT: Analysis of the genome-wide transcrptional effects caused by the deletion of the IWR1 gene by comparing the transcriptional profile of a iwr1 mutant strain with the isogenic wild type strain when cells were exponentially grown in YPD medium. Three independent cultures for the wt and the iwr1 mutant were used for the whole genome transcription analysis and they were grown in YPD at the early exponential phase. Total RNA was isolated and cDNA synthesis and labeling, filter hybridization and quantification/normalization of hybridization signals were performed as described in Garcia-Martinez, J., Aranda, A. and Perez-Ortin, J.E. (2004) Mol Cell 15(2),303-313.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Paco Estruch

PROVIDER: E-GEOD-17303 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.

Peiró-Chova Lorena L Estruch Francisco F

The Journal of biological chemistry 20090813 42

RNA polymerase II (RNA pol II) is a multisubunit enzyme that requires many auxiliary factors for its activity. Over the years, these factors have been identified using both biochemical and genetic approaches. Recently, the systematic characterization of protein complexes by tandem affinity purification and mass spectroscopy has allowed the identification of new components of well established complexes, including the RNA pol II holoenzyme. Using this approach, a novel and highly conserved factor, ...[more]

PMID: 19679657

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