Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Oogenesis in Solea senegalensis: vitellogenic versus atretic ovaries

ABSTRACT: Transcriptional profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (CerdÃ et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals and IR-At-1, IR-At-2, IR-At-3 for atretic individuals). For the experiment, the atretic stage was used as the control.

ORGANISM(S): Solea senegalensis

SUBMITTER: angela tingaud-sequeira

PROVIDER: E-GEOD-17334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis.

Tingaud-Sequeira Angèle A Chauvigné François F Lozano Juanjo J Agulleiro María J MJ Asensio Esther E Cerdà Joan J

BMC genomics 20090915

<h4>Background</h4>The Senegalese sole (Solea senegalensis) is a marine flatfish of increasing commercial interest. However, the reproduction of this species in captivity is not yet controlled mainly because of the poor knowledge on its reproductive physiology, as it occurs for other non-salmonid marine teleosts that exhibit group-synchronous ovarian follicle development. In order to investigate intra-ovarian molecular mechanisms in Senegalese sole, the aim of the present study was to identify d ...[more]

PMID: 19754951

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Project description:Background: In mammals, female fertility is determined by the outcome of follicular development (ovulation or atresia). Follicular atresia is a complex physiological process that results in the degeneration of oocytes from the ovary. However, the molecular mechanisms of oocyte degeneration and key protein markers of follicular atresia remain unclear. In this study, we explored the complex transcriptional regulatory mechanisms and protein profiles in oocytes and follicular fluid in atretic follicle stages using single-cell RNA sequencing and tandem mass tag proteomics. Results: First, through paired analysis of different follicle development stages, we identified 175 atresia-specific genes and eight candidate oocyte-secreted factors, including PKG1, YTHDF2, and MYC. Meanwhile, we also characterized unique features of the oocyte transcriptional landscape in the atretic follicle stage that displayed cell death-related transcriptional changes and mechanisms, such as autophagy (TBK1 and IRS4), necroptosis (PKR), and apoptosis (MARCKS). Moreover, we identified atresia-specific genes, namely FTH1, TF, and ACSL4, which may participate in regulation of oocyte ferroptosis in atretic follicles through a series of mechanisms including ferritinophagy, ferritin transport, and lipid metabolism. Additionally, we uncovered 333 differentially expressed proteins that may coordinate follicular atresia and revealed key pathways, such as negative regulation of angiogenesis, metabolic pathways, and transcription and mRNA splicing, that lead to oocyte degeneration. Finally, by combining transcriptome and proteomics analyses, we identified two oocyte-secreted biomarkers, PGK1 and ANGPT2, that may be associated with follicular atresia. Conclusions: In conclusion, our work provides a comprehensive characterization of oocyte degeneration in ovine atretic follicles, which provides a basis for establishing an oocyte quality evaluation system and understanding the mechanism of follicular atresia in sheep, as well as an important reference for in vitro production of embryos.

Dataset Information

Oogenesis in Solea senegalensis: vitellogenic versus atretic ovaries

Publications

New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis.

Similar Datasets

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