Transcription profiling of human hypoxia exposed normal human esophageal epithelial cells

ABSTRACT: To delineate the role of hypoxia in esophageal epithelial biology, we carried out gene array experiments using a non-transformed immortalized diploid human esophageal cell line, EPC2-hTERT (Mol Cancer Res. 2003;1:729-38). Unlike cancer cell lines, EPC2-hTERT has no genetic alterations at early passages that may affect the cellular response to hypoxia. Experiment Overall Design: EPC2-hTERT cells were exposed to moderate (1% O2) hypoxia in experiment 1 (Exp1) or severe (0.2% O2) hypoxia in experiment 2 (Exp2). Normoxia (21% O2) served as a control in both experiments.

ORGANISM(S): Homo sapiens

SUBMITTER: Hiroshi Nakagawa 

PROVIDER: E-GEOD-17353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress