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Transcription profiling of E. coli O157:H7 when exposed to sodium benzoate

ABSTRACT: Sodium benzoate is a widely used food antimicrobial in drinks and fruit juices. A microarray study was conducted to determine the transcriptional response of Escherichia coli O157:H7 to 0.5% (w/v) sodium benzoate. Stationary phase E. coli O157:H7 grown in 150 ml Luria-Bertani broth (LB) was exposed to 0 (control) and 0.5% sodium benzoate. Each treatment was duplicated and sampled at 0 (immediately after exposure), 5, 15, 30, and 60 min. Total RNA was extracted and analyzed with E. coli 2.0 Gene Chips. Experiment Overall Design: Completely randomized design with 0% (control) and 0.5% (w/v) sodium benzoate treated cells were exposed for 0 (immediately after exposure), 5 min, 15 min, 30 min, and 60 min. Each experiment was replicated once and total RNA was extracted and Affymetrix prokaryotic protocol was followed to hybridize cDNA onto E. coli Genome 2.0 Arrays.

ORGANISM(S): Escherichia coli O157:H7 EDL933

SUBMITTER: Faith Critzer

PROVIDER: E-GEOD-17519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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