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5-aza-2-deoxycytidine treatment of Swarm rat chondrosarcoma cells

ABSTRACT: The puropose of this experiment was to identify gene expression changes that result from 5-aza-2-deoxycytidine induced DNA demethylation of Swarm rat chondrosarcoma cells. The gene expression profiles of untreated Swarm rat chondrosarcoma cells were compared to the gene expression profiles of Swarm rat chondrosarcoma cells that were treated for 5 passages with a low dose of 5-aza-2-deoxycytidine (0.1uM). Five chip study. For these experiments, microarray was carried out on untreated (control) Swarm rat chondrosarcoma cells (3 biological replicates), and microarray was also carried out on Swarm rat chondrosarcoma cells treated with 5-aza-2-deoxycytidine (2 biological replicates).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Christopher Hamm 

PROVIDER: E-GEOD-17598 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global demethylation of rat chondrosarcoma cells after treatment with 5-aza-2'-deoxycytidine results in increased tumorigenicity.

Hamm Christopher A CA   Xie Hehuang H   Costa Fabricio F FF   Vanin Elio F EF   Seftor Elisabeth A EA   Sredni Simone T ST   Bischof Jared J   Wang Deli D   Bonaldo Maria F MF   Hendrix Mary J C MJ   Soares Marcelo B MB  

PloS one 20091217 12

Abnormal patterns of DNA methylation are observed in several types of human cancer. While localized DNA methylation of CpG islands has been associated with gene silencing, the effect that genome-wide loss of methylation has on tumorigenesis is not completely known. To examine its effect on tumorigenesis, we induced DNA demethylation in a rat model of human chondrosarcoma using 5-aza-2-deoxycytidine. Rat specific pyrosequencing assays were utilized to assess the methylation levels in both LINEs a  ...[more]

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