Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression analysis of a podocyte specific PTIP deletion in mouse glomerular preparations at 1 month of age


ABSTRACT: Glomerular RNA comparison between wild-type and podocyte specific deletion of the PTIP gene in 1 month old kidneys. The PTIP gene was deleted using a floxed allele and a Podocin-Cre driver strain. These mice develop protein urea by 3 months of age. This study was designed to find gene expression differences prior to the onset of the phenotype. A ptip floxed allele was crossed into the Podocin-Cre expressing strain to generate ptip fl/fl homozygous mice or ptip fl/- transheterozygous mice with or without the Cre expressing transgene. Animals were genotyped and assigend into two groups: Mutants with PTIP deletion (PTIP-) with the genotype ptip fl/fl:Pod-Cre or ptip fl/-:Pod-Cre or control mice (PTIP+) with either ptip fl/fl or ptip fl/-. Note that the floxed allele produced normal levels of wild-type PTIP protein. Kidneys were excised at 1 month of age. Glomeruli enriched fractions were generated by sieving of the tissue homogenates. RNA was prepared from glomerular enriched fractions.

ORGANISM(S): Mus musculus

SUBMITTER: Greg Dressler 

PROVIDER: E-GEOD-17709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2009-08-19 | GSE17709 | GEO
2010-08-03 | E-GEOD-20235 | biostudies-arrayexpress
2010-08-03 | GSE20235 | GEO
2010-03-06 | E-GEOD-20570 | biostudies-arrayexpress
2023-11-02 | GSE227610 | GEO
2023-09-20 | GSE184794 | GEO
2013-11-08 | GSE52234 | GEO
2013-11-08 | E-GEOD-52234 | biostudies-arrayexpress
2023-02-22 | GSE206752 | GEO
2014-09-30 | GSE42052 | GEO