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Microarray analysis of mRNAs enriched in the vegetal cortex of Xenopus oocytes

ABSTRACT: RNA localization is a fundamental mechanism for controlling the spatial regulation of protein synthesis within cells, as well as differential cell fates during early development. Localized RNAs are known to control critical aspects of early Xenopus development, but few have been studied in detail. We set out to identify novel transcripts localized to the vegetal cortex of Xenopus oocytes, one of the best-studied examples of RNA localization. We identified over 400 transcripts enriched in the vegetal cortex, compared with whole oocytes. Included were many novel genes, as well as known genes not thought to undergo RNA localization. These data suggest that the role of RNA localization in early development is extensive and will provide a resource for identifying candidate regulatory genes for early developmental processes. RNA was extracted from isolated vegetal cortices and compared with whole oocytes. Two biological replicates were used, each from a different donor female.

ORGANISM(S): Xenopus laevis

SUBMITTER: Douglas Houston 

PROVIDER: E-GEOD-17713 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA.

Cuykendall Tawny N TN   Houston Douglas W DW  

Developmental dynamics : an official publication of the American Association of Anatomists 20100601 6

RNA localization is a common mechanism for regulating cell structure and function. Localized RNAs in Xenopus oocytes are critical for early development, including germline specification by the germ plasm. Despite the importance of these localized RNAs, only approximately 25 have been identified and fewer are functionally characterized. Using microarrays, we identified a large set of localized RNAs from the vegetal cortex. Overall, our results indicate a minimum of 275 localized RNAs in oocytes,  ...[more]

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