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Expression profiling of different adult female tissues isolated from Anopheles gambiae females

ABSTRACT: Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify transcripts that are specific or enriched in circulating hemocytes compared to either neuronal or to the rest of the body. Hemocytes, heads and carcasses were isolated from naïve adult females 1-2 days after eclosion.

ORGANISM(S): Anopheles gambiae

SUBMITTER: Kristin Michel

PROVIDER: E-GEOD-17919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae.

Pinto Sofia B SB Lombardo Fabrizio F Koutsos Anastasios C AC Waterhouse Robert M RM McKay Krista K An Chunju C Ramakrishnan Chandra C Kafatos Fotis C FC Michel Kristin K

Proceedings of the National Academy of Sciences of the United States of America 20091125 50

Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. However, the molecular composition of these important immune cells has not been elucidated in depth, because of their scarcity in the circulating hemolymph, their adhesion to multiple tissues and the lack of primary culture methods to produce sufficient material for a genome-wide analysis. In this ...[more]

PMID: 19940242

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