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Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA profiles of adipocyte-derived microvesicles (ADMs)

ABSTRACT: miRNA profiles of adipocyte-derived microvesicles (ADMs) on the Day 2-4 and Day 8-10 were compared. ADMs were prepared from the 48h-conditioned medium of 3T3-L1 adipocytes (Day 2-4 and Day 8-10) followed by RNA isolation.

ORGANISM(S): Mus musculus

SUBMITTER: Naohito Aoki

PROVIDER: E-GEOD-17977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adipocyte-derived microvesicles contain RNA that is transported into macrophages and might be secreted into blood circulation.

Ogawa Rumiko R Tanaka Chie C Sato Masahiro M Nagasaki Haruka H Sugimura Kazuto K Okumura Katsuzumi K Nakagawa Yoshimi Y Aoki Naohito N

Biochemical and biophysical research communications 20100717 4

We have recently found that 3T3-L1 adipocytes secrete microvesicles, known as adipocyte-derived microvesicles (ADMs), with angiogenic activity. In this study, we found that ADMs contain RNA without typical 28S and 18S ribosomal RNA inside the vesicles. Microarray analysis revealed that ADMs contain approximately 7000 mRNAs and 140 microRNAs. Most of transcripts for adipocyte-specific and dominant genes were contained in the ADMs, and their abundance was mostly correlated with that in the donor c ...[more]

PMID: 20621060

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