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Transcription profiling of human HEK293 cells transfected with EWS-FLI1 (Ewing sarcoma family fusion gene)

ABSTRACT: Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004)

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Christoph Hutter 

PROVIDER: E-GEOD-1822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DNA microarrays reveal relationship of Ewing family tumors to both endothelial and fetal neural crest-derived cells and define novel targets.

Staege Martin S MS   Hutter Christoph C   Neumann Ingo I   Foja Sabine S   Hattenhorst Uwe E UE   Hansen Gesine G   Afar Danny D   Burdach Stefan E G SE  

Cancer research 20041101 22

Ewing family tumors (EFTs) are small round blue cell tumors that show features of neuroectodermal differentiation. However, the histogenetic origin of EFTs is still a matter of debate. We used high-density DNA microarrays for the identification of EFT-specific gene expression profiles in comparison with normal tissues of diverse origin. We identified 37 genes that are up-regulated in EFTs compared with normal tissues and validated expression of these genes in EFTs by both conventional and quanti  ...[more]

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