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Sequencing of rice degradome

ABSTRACT: miRNAs can regulate target gene expression by mRNA cleavage. Rice degradome sequencing was employed to validate mRNA targets of rice miRNAs. Two rice samples, 3-week-old seedling and young panicles were included in the study.

ORGANISM(S): Oryza sativa

SUBMITTER: Yijun Qi

PROVIDER: E-GEOD-18248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rice MicroRNA effector complexes and targets.

Wu Liang L Zhang Qingqing Q Zhou Huanyu H Ni Fangrui F Wu Xueying X Qi Yijun Y

The Plant cell 20091110 11

MicroRNAs (miRNAs) are small silencing RNAs with regulatory roles in gene expression. miRNAs interact with Argonaute (AGO) proteins to form effector complexes that cleave target mRNAs or repress translation. Rice (Oryza sativa) encodes four AGO1 homologs (AGO1a, AGO1b, AGO1c, and AGO1d). We used RNA interference (RNAi) to knock down the four AGO1s. The RNAi lines displayed pleiotropic developmental phenotypes and had increased accumulation of miRNA targets. AGO1a, AGO1b, and AGO1c complexes were ...[more]

PMID: 19903869

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Profiling of small RNA populations in rice total extract and purified AGO1 complexes

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