Unknown,Transcriptomics,Genomics,Proteomics

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CREB ChIP-chip and HaloCHIP-chip experiments


ABSTRACT: Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells. CREB ChIP-chip two biologcal replicates. HaloCHIP-chip three biological replicates with and without Forskolin

ORGANISM(S): Homo sapiens

SUBMITTER: Danette Hartzell 

PROVIDER: E-GEOD-18347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.

Hartzell Danette D DD   Trinklein Nathan D ND   Mendez Jacqui J   Murphy Nancy N   Aldred Shelley F SF   Wood Keith K   Urh Marjeta M  

BMC genomics 20091027


<h4>Background</h4>Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHI  ...[more]

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