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Expression analysis of Xylella fastidiosa PD Temecula strain grown in different media

ABSTRACT: Investigation of whole genome gene expression level changes in Xylella fastidiosa grown in minimal media XFM and XFM supplied with pectin or glucan (Host polysaccharides) , compared to cell grown in the complex media PWG. The cells grown in the minimal medium XFM supplied with host polysaccharides specially pectin are transmissible by the insect vector when delivered to the vector through artificial diet system. This does not happen with cells grown in the complex media. 4 (4 plex chips) study using total RNA recovered from 4 independents replicates for Xylella fastidiosa grown on PWG, XFM, XFM-glucan and XFM-pectin.

ORGANISM(S): Xylella fastidiosa Temecula1

SUBMITTER: Rodrigo Almeida

PROVIDER: E-GEOD-18369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Host structural carbohydrate induces vector transmission of a bacterial plant pathogen.

Killiny Nabil N Almeida Rodrigo P P RP

Proceedings of the National Academy of Sciences of the United States of America 20091215 52

Many insect-borne pathogens have complex life histories because they must colonize both hosts and vectors for successful dissemination. In addition, the transition from host to vector environments may require changes in gene expression before the pathogen's departure from the host. Xylella fastidiosa is a xylem-limited plant-pathogenic bacterium transmitted by leafhopper vectors that causes diseases in a number of economically important plants. We hypothesized that factors of host origin, such a ...[more]

PMID: 20018775

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Expression analysis of Xylella fastidiosa PD Temecula strain grown in different media

Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa grown in minimal media XFM and XFM supplied with pectin or glucan (Host polysaccharides) , compared to cell grown in the complex media PWG. The cells grown in the minimal medium XFM supplied with host polysaccharides specially pectin are transmissible by the insect vector when delivered to the vector through artificial diet system. This does not happen with cells grown in the complex media.

2009-10-03 | GSE18369 | GEO

New insights on resistance mechanisms to antimicrobial compounds in a plant pathogen biofilm

Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa 9a5c biofilm, submitted to treatments with sub inhibitory and inhibitory concentrations of copper and tetracycline. A study of Xylella fastidiosa 9a5c was done using total RNA recovered from biofilm bacterial cells submitted to 3 or 7mM of CuSO4 or 100 or 800 µg/ml of tetracycline. Each chip measures the expression level of 2832 genes from Xylella fastidiosa 9a5c with thirteen 60-mer probe pairs (PM/MM) per gene, with five-fold technical redundancy.

2010-08-31 | E-GEOD-21645 | biostudies-arrayexpress

Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T grown in mono- and co-cultures on xylan or pectin

Project description:Rumen bacterial species belonging to the genera Butyrivibrio are important degraders of plant polysaccharides, particularly hemicelluloses (arabinoxylans) and pectin. Currently, four distinct species are recognized which have very similar substrate utilization profiles, but little is known about how these microorganisms are able to co-exist in the rumen. To investigate this question, Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T were grown alone or in co-culture on the insoluble substrates, xylan or pectin, and their growth, release of sugars, fermentation end products and transcriptomes were examined. In single cultures, B316T was able to degrade and grow well on xylan and pectin, while MB2003 was unable to utilize either of these insoluble substrates to support significant growth. Co-cultures of B316T grown with MB2003 revealed that MB2003 showed almost equivalent growth to B316T when either xylan or pectin were supplied as substrates. The effect of co-culture on the transcriptomes of B316T and MB2003 was very marked; B316T transcription was largely unaffected by the presence MB2003, but MB2003 expressed a wide range of genes encoding carbohydrate degradation/metabolism and oligosaccharide transport/assimilation in order to compete with B316T for the released sugars. These results suggest that B316T has a role as an initiator of the primary solubilization of xylan and pectin, while MB2003 competes effectively as a scavenger for the released soluble sugars to enable its growth and maintenance in the rumen.

2018-09-27 | GSE120544 | GEO

New insights on resistance mechanisms to antimicrobial compounds in a plant pathogen biofilm

2010-08-31 | GSE21645 | GEO

Comparative genomic hybridization of Xylella fastidiosa 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic)

Project description:Comparison of genomic DNA from two strains of Xylella fastidiosa: 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic).

2004-04-01 | E-MEXP-22 | biostudies-arrayexpress

Xylella fastidiosa Temecula1

Project description:Expression analysis of Xylella fastidiosa PD Temecula strain grown in different media

| PRJNA118133 | ENA

Heat Shock response of the phytopathogenic bacteria Xylella fastidiosa

Project description:This is the study of the Heat Shock response of phytopathogenic bacteria Xylella fastidiosa. This series keeps the 25 minutes 40oC stimulus response (Aug 2005). Keywords: stress response; heat shock response

2006-06-01 | GSE3044 | GEO

Xylella fastidiosa

Project description:Xylella fastidiosa Study on different growth media

| PRJNA109403 | ENA

Using next generation sequencing to compare gene expression of wild type and Δcsp1 mutant of bacterial plant pathogen Xylella fastidiosa strain Stag's Leap

Project description:To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing.

2021-10-10 | GSE184594 | GEO

Xylella fastidiosa 9a5c: gomesin-treated cells vs. control

Project description:In the xylem vessels of susceptible hosts, such as citrus trees or grapevines, Xylella fastidiosa forms biofilm like-colonies that can block water transport, which appears to correlate to disease symptoms. Besides helping host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMP). Here we show that gomesin, a potent AMP from a Brazilian tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min treatment with a sublethal concentration. Data from DNA microarray hybridizations revealed that among the up-regulated coding sequences (CDS), some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by non-treated cells or cells exposed to streptomycin. We have also observed that treatment of X. fastidiosa with sublethal concentration of gomesin before inoculation in tobacco plants correlates with reduction in CVC symptoms, an effect possibly due to trapping of bacterial cells to fewer xylem vessels given the enhancement in biofilm production. Together, our results suggest that X. fastidiosa can selectively sense a sublethal concentration of gomesin modulating its gene expression to produce a stronger biofilm that may protect itself against the toxic effects of this AMP. Two-condition experiment, control (PW media) vs. gomesin-treated cells. Biological replicates: 5 control, 5 gomesin-treated, independently grown and harvested. Two replicates (left and right side of slide) per array. Total of 10 slides were hibridized, including dye-swap of and two self-self experiments.

2010-05-16 | E-GEOD-17605 | biostudies-arrayexpress

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