Transcriptome analysis of monocytic leukemia cell differentiation
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: David Piquemal
PROVIDER: E-GEOD-1865 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transcriptome analysis of monocytic leukemia cell differentiation.
Piquemal David D Commes Thérèse T Manchon Laurent L Lejeune Mireille M Ferraz Conchita C Pugnère Denis D Demaille Jacques J Elalouf Jean-Marc JM Marti Jacques J
Genomics 20020901 3
The human leukemia cell line U937 is a well-established model for studying monocytic cell differentiation. We used a modified protocol (SADE) of serial analysis of gene expression (SAGE) and developed a SADE linker-anchored PCR assay to investigate the pattern of expression of known genes and to identify new transcripts in proliferating cells and during cell growth arrest and differentiation. We implemented new informatic tools to compare expression profiles before and after exposure of cells to ...[more]
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