Response to nicotine in heads of the tobacco aphid Myzus persicae
Ontology highlight
ABSTRACT:
ORGANISM(S): Myzus persicae
SUBMITTER: John Ramsey
PROVIDER: E-GEOD-18659 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Response of the aphid head transcriptome to nicotine in artificial diets (250 µM nicotine, 24 hours feeding) Two condition experiment: heads of aphids feeding on control diet vs heads of aphids feeding on 250 µM nicotine containing diet
2010-05-14 | E-GEOD-18658 | biostudies-arrayexpress
Project description:Response of the aphid head transcriptome to nicotine in artificial diets (100 µM nicotine, 24 hours feeding) Two condition experiment: heads of aphids feeding on control diet vs heads of aphids feeding on 100 µM nicotine containing diet
2010-05-14 | E-GEOD-18657 | biostudies-arrayexpress
Project description:The green peach aphid/peach-potato aphid Myzus persicae can colonize hundreds of plant species, an ability that is in part due to the delivery of saliva proteins – often referred to as effectors – into the host plant that suppress plant defence. As a generalist herbivore with a remarkable ability to colonize new host plants M. persicae represents an outstanding model system for studying the molecular mechanisms underlying plant-insect interactions. Recent advancements in mass spectrometry instrumentation and database search software along with a new high-quality reference genome assembly for M. persicae and a simplified method for improved aphid saliva recovery, collectively enhance the detection of saliva proteins with unprecedented sensitivity and specificity.
2024-08-28 | PXD055051 | Pride
Project description:The aim of this experiment was to compare the transciptome of the peach-potato aphid (Myzus persicae) clone 4106a (a laboratory insecticide-susceptible standard collected from potato in Scotland in 2000) with clone 5191A (an insecticide resistant aphid clone collected from tobacco in Greece in 2007) to identify which genes are over or underexpressed in the resistant phenotype. Two-condition experiment, 4106a vs. 5191a Myzus persicae clones. Biological replicates: 4 pools of RNA extracted from ten 15 day old aphids of each clone. Technical Replicates: Two technical reps incorporating a dye swap. Total replication: eight replicates for each clone.
2010-10-07 | E-GEOD-20542 | biostudies-arrayexpress
Project description:In this study we used a multidisciplinary proteomics approach combining LC-ESI-MS/MS and 2D-DIGE/MALDI-TOF/MS to compare the salivary proteins from three aphid species including Acyrthosiphon pisum, Megoura viciae, and Myzus persicae. Comparative analyses revealed variability among aphid salivary proteomes. Among the proteins that varied 22% were related to DNA-binding, 19% were related to GTP-binding, and 19% had oxidoreductase activity.
2017-08-17 | PXD000476 | Pride
Project description:Response to nicotine (100 µM) in heads of the tobacco aphid Myzus persicae
| PRJNA123821 | ENA
Project description:Response to nicotine (250 µM) in heads of the tobacco aphid Myzus persicae
| PRJNA123823 | ENA
Project description:Aphids, sap-sucking insects in the order Hemiptera, are among the most prolific insect vectors of plant viruses Plant viruses from the family Luteoviridae are transmitted exclusively by aphids in a circulative manner and cause significant crop yield losses. Circulative plant viruses must cross the aphid gut and other tissues prior to transmission to a new host plant. The discovery of proteins that control acquisition and transmission in the insect vector is the biggest challenge for the vector biology field and will have practical applications for growers by providing new molecular targets for the development of precision vector management tools. The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV), a polerovirus in the Luteoviridae. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip (T-Myzus) as compared to the weed physalis (P-Myzus). The effect on PLRV transmission efficiency was transient and caused by a host-switch response. Using 2-D DIGE, we revealed that the major difference in the proteome profile of P- and T-Myzus was the lysosomal cysteine protease cathepsin B, with multiple size and charge isoforms of this enzyme up-regulated in T-Myzus. Quantitative, shotgun proteomics revealed a specific upregulation in the expression of other lysosomal proteins in T-Myzus as compared to P-Myzus, including cathepsin B, cathepsin B-16, beta-glucuronidase, peroxidasin, legumain-like, and aminopeptidase-N. The titer of PLRV was over 1.5 fold higher in P-Myzus than in T-Myzus at 24h and 72h after the beginning of virus acquisition, suggesting that virus acquisition in P-Myzus was more efficient. Cathepsin B and PLRV localization were starkly different in P- and T-Myzus midguts, the site of PLRV acquisition into the insect. In P-Myzus midguts, an abundance of PLRV was observed inside midgut cells, and cathepsin B was sequestered in a subcellular compartment. In contrast, there is near complete co-localization of cathepsin B and PLRV at the cell membranes in viruliferous T-Myzus. Inhibition of cathepsin and other cysteine proteases with E64 restored the ability of T-Myzus to transmit PLRV in a dose-dependent manner, suggesting that the activities of lysosomal cysteine proteases at the cell membrane in T-Myzus is responsible for the change in virus transmission phenotype in these aphids. T-Myzus individuals weighed more and had more progeny than P-Myzus individuals. These data are all consistent with the hypothesis that there is an induction of lysosomal exocytos in the midgut of T-Myzus linked to the ability of the aphid to acquire PLRV. These data also show that the ability of the generalist aphid M. persicae to transmit PLRV is influenced by the host plant the aphids are reared on, information that is useful to growers for polerovirus management in field crops.
2016-12-14 | PXD004893 | Pride
Project description:The aim of this experiment was to compare the transciptome of the peach-potato aphid (Myzus persicae) clone 4106a (a laboratory insecticide-susceptible standard collected from potato in Scotland in 2000) with clone 5191A (an insecticide resistant aphid clone collected from tobacco in Greece in 2007) to identify which genes are over or underexpressed in the resistant phenotype.
2010-10-07 | GSE20542 | GEO
Project description:Myzus persicae (green peach aphid) feeding on Arabidopsis thaliana induces a defense response, quantified as reduced aphid progeny production, in infested leaves but not in other parts of the plant. Similarly, infiltration of aphid saliva into Arabidopsis leaves causes only a local increase in aphid resistance. Further characterization of the defense-eliciting salivary components indicates that Arabidopsis recognizes a proteinaceous elicitor with a size between 3 to 10 kD. Genetic analysis using well-characterized Arabidopsis mutant shows that saliva-induced resistance against M. persicae is independent of the known defense signaling pathways involving salicylic acid, jasmonate, and ethylene. Among 78 Arabidopsis genes that were induced by aphid saliva infiltration, 52 had been identified previously as aphid-induced, but few are responsive to the well-known plant defense signaling molecules salicylic acid and jasmonate. Quantitative PCR analysis confirms expression of saliva-induced genes. In particular, expression of a set of O-methyltransferases, which may be involved in the synthesis of aphid-repellent glucosinolates, was significantly up-regulated by both M. persicae feeding and treatment with aphid saliva. However, this did not correlate with increased production of 4-methoxyindol-3-ylmethylglucosinolate, suggesting that aphid salivary components trigger an Arabidopsis defense response that is independent of this aphid-deterrent glucosinolate. Experiment Overall Design: 3 biological replicates (control and treatment). Total number of samples: 6.
2009-06-23 | E-GEOD-16497 | biostudies-arrayexpress