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Expression data from two pair of osteosarcoma sublines with difeerental pulmonary

ABSTRACT: Metastasis of tumor consists of a complex serias of cascades . Molecular mechanisms needs to be elucidated. We used microarrays to explore the global change of gene expression betwwen two pairs of osteosarcoma sublines screened by different method. The assay was performed among three pairs of cublines, the first two pairs of sublines comes from the different passage of sublines established with orthotopic transplantation under the established cell line named Sosp-9607 ,the other pair was screened by the tail-vein injection method of commercial avaliable cell line-Saos-2.

ORGANISM(S): Homo sapiens

SUBMITTER: xiang chen

PROVIDER: E-GEOD-18947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from two pair of osteosarcoma sublines with difeerental pulmonary

Project description:Metastasis of tumor consists of a complex serias of cascades . Molecular mechanisms needs to be elucidated. We used microarrays to explore the global change of gene expression betwwen two pairs of osteosarcoma sublines screened by different method.

2009-11-10 | GSE18947 | GEO

Human Embryonic Stem Cell Sublines

Project description:Human embryonic stem (ES) cell lines acquire recurrent karyotypic abnormalities that may promote growth and survival in long-term culture. Cells with these abnormalities are often present at low levels in human ES cell cultures, requiring subcloning for isolation and analyses. We have developed a rapid method to isolate either abnormal or normal clones from karyotypically mosaic human ES cell cultures that we call Genetic Diagnosis at Passage (G-DAP). We have used this technique to isolate three distinct abnormal sublines from H9 human ES cells in continuous culture. These sublines were characterized by G-banding, fluorescence in situ hybridization (FISH), spectral karyotyping (SKY), and array comparative genomic hybridization (aCGH). One subline (H9.DT) has, as it’s only abnormality, a duplication of 12p capped by a 5q subtelomere. A related subline (H9.TSF) has an additional copy of the derivative chromosome 12, partial trisomy of 17q, and trisomy 14. The third subline (H9.DE) has an interstitial deletion of 18q as the sole anomaly. Despite these karyotypic abnormalities, all three sublines retain markers of the undifferentiated state. The subline H9.TSF demonstrated the ability to form embryoid bodies consisting of all three germ layers. Significantly, H9.DT and H9.TSF sublines show a classic karyotype progression that mirrors that seen in both hematopoietic and solid tumors. This progression was accompanied by an impressive increase in growth potential. The isolation of human ES cell sublines with clonal cytogenetic abnormalities provides a valuable tool for study of factors that promote in vitro genetic changes and for analysis of mechanisms of aneuploidy related to genetic progression found in human cancers.

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MicroRNAs among esophageal cancer cell sublines with different migration ability

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Expression data among esophageal cancer cell sublines with different migration ability

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Project description:The orthotopic transplantation of human OEC-M1 cells in immune-compromised mice was established to feasibly study tumorigenesis and lymph node metastasis of OSCC. Through in vivo selection, two sublines, designated as LN1-1 and LN1-2 were successfully isolated from cervical lymph nodes and identified to originate from OEC-M1 cells.

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Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

Project description:Gene expression profiles of in vitro selected highly metastatic MKN45-GFP sublines. The results were compared with MKN45-GFP control cell line to determine the metastasis associated genes. Four pairs compared experiment. Each pair was used MKN45-GFP cells as correlated control. Determining on the gene expression trends were by various metastatic ability of each subline.

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Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines

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MicroRNAs among esophageal cancer cell sublines with different migration ability

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2010-06-16 | E-GEOD-17188 | biostudies-arrayexpress

Gene expression analysis of OVCAR-3 and CDK2 resistant sublines

Project description:Cyclin E1 (CCNE1) is amplified in various tumor types including high-grade serous ovarian cancer where it is associated with poor clinical outcome. We have demonstrate that suppression of the Cyclin E1 partner kinase, CDK2, induces apoptosis in a CCNE1 amplicon-dependent manner. Little is known of mechanisms of resistance to CDK inhibitors. We therefore generated OVCAR-3 sublines with reduced sensitivity to CDK2 inhibitors and profiled by gene expression microarrays. Arrayed samples included parental OVCAR-3 cells (n = 4 replicates) and five independently derived sublines resistant to PHA-533533 (OVCAR3-533533-R1, -R3, -R5, -R6, -R7). The resistant cell lines were arrayed after drug selection (P5) and after continued passage in the absence of inhibitor (P10+).

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