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Genome-wide mapping of Nr5a2 in mouse embryonic stem cells

ABSTRACT: Nr5a2 (also known as liver receptor homolog-1, Lrh-1) has been shown to bind both the proximal enhancer and proximal promoter regions of Pou5f1 and regulate Pou5f1 in the epiblast stage of mouse embryonic development (Gu et al., 2005). Nr5a2-null embryos display a loss of Oct4 expression in the epiblasts (Gu et al., 2005) and die between E6.5 and E7.5 (Gu et al., 2005; Pare et al., 2004). To identify the targets of Nr5a2, we generated a stable ES cell-line that expresses HA-tagged Nr5a2. Anti-HA antibody was used to immunoprecipitate HA-Nr5a2 for ChIP-seq analysis. Keywords: Transcription factor binding sites To identify the binding sites of Nr5a2, we generated a stable ES cell-line that expresses HA-tagged Nr5a2. Anti-HA antibody was used to immunoprecipitate HA-Nr5a2.

ORGANISM(S): Mus musculus

SUBMITTER: Mikael Huss 

PROVIDER: E-GEOD-19019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to pluripotent cells.

Heng Jian-Chien Dominic JC   Feng Bo B   Han Jianyong J   Jiang Jianming J   Kraus Petra P   Ng Jia-Hui JH   Orlov Yuriy L YL   Huss Mikael M   Yang Lin L   Lufkin Thomas T   Lim Bing B   Ng Huck-Hui HH  

Cell stem cell 20100121 2

Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) with the introduction of Oct4, Sox2, Klf4, and c-Myc. Among these four factors, Oct4 is critical in inducing pluripotency because no transcription factor can substitute for Oct4, whereas Sox2, Klf4, and c-Myc can be replaced by other factors. Here we show that the orphan nuclear receptor Nr5a2 (also known as Lrh-1) can replace Oct4 in the derivation of iPSCs from mouse somatic cells, and it can also enhance reprogramming  ...[more]

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