Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

34 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pichia angusta cells glucose- vs methanol-grown

ABSTRACT: Transcriptional profiling of Pichia angusta NCYC 495 leu1.1 cells growing exponentially on mineral medium containing 0.5% glucose versus a shift for 2 hours to mineral medium containing 0.5% methanol. Two-conditions experiment, 4 individual glucose-grown cultures were shifted at OD660nm 2.3 to fresh mineral medium OD 0.2 containing 0.5% methanol for 2 hours. Transcriptional profiling was performed using total RNA from the glucose-grown cells at the moment of the shift versus cells adapted to methanol for 2 hours.

ORGANISM(S): Pichia angusta NCYC 495 leu1.1

SUBMITTER: Ida van der Klei

PROVIDER: E-GEOD-19036 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis.

van Zutphen Tim T Baerends Richard J S RJ Susanna Kim A KA de Jong Anne A Kuipers Oscar P OP Veenhuis Marten M van der Klei Ida J IJ

BMC genomics 20100104

<h4>Background</h4>Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarge ...[more]

PMID: 20044946

Similar Datasets

Pichia angusta cells glucose- vs methanol-grown

Project description:Transcriptional profiling of Pichia angusta NCYC 495 leu1.1 cells growing exponentially on mineral medium containing 0.5% glucose versus a shift for 2 hours to mineral medium containing 0.5% methanol.

2009-11-17 | GSE19036 | GEO

Pichia pastoris Glucose/Methanol comparison

2015-08-31 | GSE67690 | GEO

N-acetylgalactosamine Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae

Project description:Three Microarray comparisons have been preformed in this study. 1- Transcriptome comparison of the Streptococcus pneumoniae D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) to M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372597 and GSM2372598). 2- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔagaR to D39 wild type grown in M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372599 and GSM2372600). 3- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) (GSM2290636 and GSM2290637).

2016-11-02 | GSE86008 | GEO

Pichia pastoris Glucose/Methanol comparison

Project description:Comparison of transcription profile of Pichia pastoris cells grown on Glucose medium with Pichia pastoris cells grown on Methanol/Glycerol medium, the fermentations were done in a chemostat. 2 color experiment in reference design. Pichia pastoris reference mix [mixed pool of Pichia pastoris cells sampled from various conditions including cells grown on glycerine, glucose and methanol, on full andminimal medium, in stationary and exponential growth phase, and in different stress states]

2015-08-31 | E-GEOD-67690 | biostudies-arrayexpress

Transcriptional response of Streptococcus pneumoniae D39 to Lactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium + 0.5 % (w/v) Lactose (LM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17).

2014-06-20 | GSE58181 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to Galactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium + 0.5 % (w/v) Galactose (GaM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17).

2014-06-20 | GSE58180 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to Cellobiose at time point T2

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium+ 0.5 % (w/v) Cellobiose (CM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17) hervasted at time point T2.

2013-02-20 | GSE43344 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to Cellobiose at time point T1

2013-02-20 | GSE43343 | GEO

To identify novel targets of a zinc finger transcription factor, Mxr1p in Pichia pastoris cultured in medium containing ethanol

Project description:The role of transcription factor Mxr1p has so far been studied in methanol, amino acid and acetate metabolism. We have demonstrated a key role of Mxr1p in regulation of ethanol metabolism when cells are cultured in medium containing ethanol as sole carbon source. We employed RNA Seq to identify new targets of Mxr1p in medium containing ethanol.

2021-09-22 | GSE168677 | GEO

Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum

Project description:Background: Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. Results: The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by genetic adaption in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine sulfhydrolase MetY and the other leading to shortened CoA transferase Cat (Q342*). Introduction of either mutation into the genome of C. glutamicum wild type increased methanol tolerance and introduction of both mutations into C. glutamicum was sufficient to achieve methanol tolerance almost indistinguishable from that of strain Tol1. Conclusion: The methanol tolerance of C. glutamicum can be increased by two point mutations leading to amino acid exchange of O-acetylhomoserine sulfhydrolase MetY and shortened CoA transferase Cat. Introduction of these mutations into producer strains may be helpful when using carbon sources containing methanol as component or impurity. The gene expression was analyzed in the methanol tolerant strain Tol1 in comparison to the C. glutamicumWT. Direct comparison in LB complex medium and analysis of expression response to methanol addition in mCGXII minimal medium with 100 mM glucose.

2015-08-01 | E-GEOD-71590 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data