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Transcription profiling of mouse RAW 264.7 macrophages treated with carbon monoxide and lipopolysaccharide

ABSTRACT: Murine RAW 264.7 peritoneal macrophages were treated with 250 ppm Carbon monoxide (CO) for 3 hours prior to the addition of LPS (10 ng/ml) and then for the subsequent 4 hours of the study. Air-treated cells were maintained for the same time without CO. Total RNA was harvested at 0, 15, 30, 60, 120 and 240 min after LPS addition. CRNA was produced using standard Affymetrix procedures from 10µg of total RNA. The expression values of all probe sets are scaled to a target intensity of 500 by using Affymetrix Microarray Suite 5.0

ORGANISM(S): Mus musculus

SUBMITTER: Martin Bilban 

PROVIDER: E-GEOD-1906 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Carbon monoxide (CO) suppresses proinflammatory responses in macrophages reacting to LPS. We hypothesize that CO acts by inducing a molecule(s) that suppresses the inflammatory response to subsequent stress. Exposure of macrophages to CO alone in vitro produced a brief burst of mitochondrial-derived ROS, which led to expression of PPARgamma. PPARgamma expression proved essential for mediating the anti-inflammatory effects of CO. Blocking the CO-mediated increase in ROS generation prevented PPARg  ...[more]

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