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Transcription profiling time series IFH1 overexpression by galactose induction in S. cerevisiae


ABSTRACT: Experiment Design: Type of experiment: time course after GAL (galactose) induction. Experimental factor: time, genotype, Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each),Samples used, extract preparation and labeling: The origin of the biological sample: Species: S. cerevisiae,Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa),UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa), Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2%, Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Stephan Schawalder 

PROVIDER: E-GEOD-1934 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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