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Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers

ABSTRACT: To delineate epithelial subpopulations in mouse mammary tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from mammary glands using fluorescence-activated cell sorting. The resultant Lin- population was fractionated into four distinct subpopulations using antibodies against CD29, CD24 and CD61. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as fibroblast-containing stromal (CD29loCD24-), mammary stem cell (MaSC)-enriched (CD29hiCD24+CD61+), luminal progenitor (CD29loCD24+CD61+), and mature luminal (CD29loCD24+CD61-) cell subpopulations. Microarray profiling was used to derive gene expression signatures of these 4 subpopulations. The four mammary cell subpopulations were found to have distinct gene expression profiles. Four mammary cell subpopulations from 3-5 pooled mouse tissues were analysed. MS is for the MaSC-enriched cell subpopulation. LP is for the Luminal Progenitor subpopulation. ML is for the Mature Luminal subpopulation.

ORGANISM(S): Mus musculus

SUBMITTER: Di Wu 

PROVIDER: E-GEOD-19446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analyses of mouse and human mammary cell subpopulations reveal multiple conserved genes and pathways.

Lim Elgene E   Wu Di D   Pal Bhupinder B   Bouras Toula T   Asselin-Labat Marie-Liesse ML   Vaillant François F   Yagita Hideo H   Lindeman Geoffrey J GJ   Smyth Gordon K GK   Visvader Jane E JE  

Breast cancer research : BCR 20100326 2

<h4>Introduction</h4>Molecular characterization of the normal epithelial cell types that reside in the mammary gland is an important step toward understanding pathways that regulate self-renewal, lineage commitment, and differentiation along the hierarchy. Here we determined the gene expression signatures of four distinct subpopulations isolated from the mouse mammary gland. The epithelial cell signatures were used to interrogate mouse models of mammary tumorigenesis and to compare with their no  ...[more]

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