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Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana

ABSTRACT: FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88. Ten-day old wild-type and flp-1 myb88 Arabidopsis green tissues were used for ChIP. Rabbit polyclonal antibody recognizing FLP and MYB88 proteins was used for chromatin immunoprecipitation

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Zidian Xie

PROVIDER: E-GEOD-19763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of cell proliferation in the stomatal lineage by the Arabidopsis MYB FOUR LIPS via direct targeting of core cell cycle genes.

Xie Zidian Z Lee Eunkyoung E Lucas Jessica R JR Morohashi Kengo K Li Dongmei D Murray James A H JA Sack Fred D FD Grotewold Erich E

The Plant cell 20100730 7

Stomata, which are epidermal pores surrounded by two guard cells, develop from a specialized stem cell lineage and function in shoot gas exchange. The Arabidopsis thaliana FOUR LIPS (FLP) and MYB88 genes encode closely related and atypical two-MYB-repeat proteins, which when mutated result in excess divisions and abnormal groups of stomata in contact. Consistent with a role in transcription, we show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other kno ...[more]

PMID: 20675570

Similar Datasets

Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana

Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88.

2010-09-01 | GSE19763 | GEO

Deceleration of the cell cycle underpins a switch from proliferative to terminal division in plant stomatal lineage

Project description:We have previously identified genes that are induced by MUTE, key transcription factor for stomatal lineage fate commitment and symmetric cell division that create stomata surrounded by a pair of guard cells in Arabidopsis. To test whether these genes are indeed direct MUTE targets, we performed genome-wide MUTE ChIP-sequencing.

2022-02-12 | GSE173338 | GEO

Microarray analysis of scap1 mutant and wild type in Arabidopsis thaliana guard cell protoplast

Project description:Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.

2013-02-28 | GSE43964 | GEO

Microarray analysis of scap1 mutant and wild type in Arabidopsis thaliana guard cell protoplast

2013-02-28 | E-GEOD-43964 | biostudies-arrayexpress

Expression data from Arabidopsis leaves

Project description:Flavonols are a class of flavonoids. Unlike other classes of flavonoids, flavonols strongly accumulate in guard cells, but the role of their accumulation in guard cells is still unclear so far.The overexpression of TaFLS1, a flavonol synthase gene from wheat, improves flavonol content in guard cells of Arabidopsis, while the mutant fls1-3 of Arabidopsis AtFLS1 reduces the content. Both the increase of flavonols by TaFLS1 overexpression and the decrease in fls1-3 change stomatal apeture, showing the association between flavonols and stomatal movements. We used microarrays to detail the global programme of gene expression underlying both the increase and decrease of flavonol content and identified possible mechanism of flavonols-mediated stomatal movement.

2014-02-01 | GSE54593 | GEO

Effect of photoperiod on gene expressions in guard cells.

Project description:To clarify the molecular mechanism in photoperiod-dependent regulation of stomatal movements, we performed RNA sequences in isolated guard cell protoplasts from Arabidopsis thaliana.

2019-07-22 | GSE104436 | GEO

Expression data from Arabidopsis leaves

2014-02-01 | E-GEOD-54593 | biostudies-arrayexpress

AtMYB60

Project description:Stomatal guard-cells modulate gas exchange between the plant and the atmosphere. Regulation of transcription is emerging as an important mechanism in controlling guard cells activity. The Arabidopsis transcription factor AtMYB60, is specifically expressed in guard cells and controls stomatal movements. Opening of stomatal pores is constitutively reduced in the atmyb60-1 null allele, and water loss during drought is diminished in the mutant compared to wild type. To address the effect of the AtMYB60 disruption on global gene expression, total mRNAs, derived from atmyb60-1 and WT rosette leaves, grown in standard conditions, were hybridized to a cDNA microarray.

2005-01-10 | E-MEXP-225 | biostudies-arrayexpress

Distinct guard cell specific remodeling of chromatin accessibility during abscisic acid and CO2 dependent stomatal regulation (RNA-Seq)

Project description:In plants, epidermal guard cells integrate and respond to numerous environmental signals to control stomatal pore apertures thereby regulating gas exchange. Chromatin structure controls transcription factor access to the genome, but whether large-scale chromatin remodeling occurs in guard cells during stomatal movements, and in response to the hormone abscisic acid (ABA) in general, remain unknown. Here we isolate guard cell nuclei from Arabidopsis thaliana plants to examine whether the physiological signals, ABA and CO2, regulate guard cell chromatin during stomatal movements. Our cell type specific analyses uncover patterns of chromatin accessibility specific to guard cells and define novel cis-regulatory sequences supporting guard cell specific gene expression. We find that ABA triggers extensive and dynamic chromatin remodeling in guard cells, roots, and mesophyll cells with clear patterns of cell-type specificity. DNA motif analyses uncover binding sites for distinct transcription factors enriched in ABA-induced and ABA-repressed chromatin. We identify the ABF/AREB bZIP-type transcription factors that are required for ABA-triggered chromatin opening in guard cells and implicate the inhibition of a set of bHLH-type transcription factors in controlling ABA-repressed chromatin. Moreover, we demonstrate that ABA and CO2 induce distinct programs of chromatin remodeling. We provide insight into the control of guard cell chromatin dynamics and propose that ABA-induced chromatin remodeling primes the genome for abiotic stress resistance.

2023-12-19 | GSE243472 | GEO

Distinct guard cell specific remodeling of chromatin accessibility during abscisic acid and CO2 dependent stomatal regulation (ATAC-Seq)

2023-12-19 | GSE243470 | GEO