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Transcription profiling of primary rat hippocampal culture cells overexpressing an activated form of calcineurin

ABSTRACT: Micorarray analysis was performed on RNA samples from hippocampal cultures infected with either Ad-aCaN or Ad-LacZ or uninfected. Each sample was applied to its own GeneChip (Rat RG-U34A; n=7-9 chips/group). Chips were then processed and scanned using Agilent Affymetrix GeneArray Scanner. MAS5 was used to determine signal intensity and presence/absence calls for the data.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Eric Blalock

PROVIDER: E-GEOD-1978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Calcineurin triggers reactive/inflammatory processes in astrocytes and is upregulated in aging and Alzheimer's models.

Norris Christopher M CM Kadish Inga I Blalock Eric M EM Chen Kuey-Chu KC Thibault Veronique V Porter Nada M NM Landfield Philip W PW Kraner Susan D SD

The Journal of neuroscience : the official journal of the Society for Neuroscience 20050501 18

Astrocyte reactivity (i.e., activation) and associated neuroinflammation are increasingly thought to contribute to neurodegenerative disease. However, the mechanisms that trigger astrocyte activation are poorly understood. Here, we studied the Ca2+-dependent phosphatase calcineurin, which regulates inflammatory signaling pathways in immune cells, for a role in astrogliosis and brain neuroinflammation. Adenoviral transfer of activated calcineurin to primary rat hippocampal cultures resulted in pr ...[more]

PMID: 15872113

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