Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Bmi1-null c-Kit+Sca-1+Lineage marker- (KSL) hematopoietic stem/progenitor cells


ABSTRACT: Bmi1 is a component of polycomb repressive complex 1 and its role in the inheritance of the stemness of adult somatic stem cells has been well characterized. Bmi1 maintains the self-renewal capacity of adult stem cells, at least partially, by repressing the Ink4a/Arf locus that encodes a cyclin-dependent kinase inhibitor, p16Ink4a, and a tumor suppressor, p19Arf 14. Deletion of both Ink4a and Arf in Bmi1-deficient mice substantially restored the defective self-renewal capacity of HSCs and neural stem cells. Purified KSL cells from BM of wild-type, Bmi1-/-, Ink4a-/-Arf-/-, and Bmi1-/- Ink4a-/-Arf-/- mice were subjected to RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: ATSUSHI IWAMA 

PROVIDER: E-GEOD-19796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Poised lineage specification in multipotential hematopoietic stem and progenitor cells by the polycomb protein Bmi1.

Oguro Hideyuki H   Yuan Jin J   Ichikawa Hitoshi H   Ikawa Tomokatsu T   Yamazaki Satoshi S   Kawamoto Hiroshi H   Nakauchi Hiromitsu H   Iwama Atsushi A  

Cell stem cell 20100301 3


Polycomb group (PcG) proteins are essential regulators of stem cells. PcG and trithorax group proteins mark developmental regulator gene promoters with bivalent domains consisting of overlapping repressive and activating histone modifications to keep them poised for activation in embryonic stem cells. Bmi1, a component of PcG complexes, maintains the self-renewal capacity of adult stem cells, but its role in multipotency remains obscure. Here we show that Bmi1 is critical for multipotency of hem  ...[more]

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