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Gene Expression profiles of E.coli grown in LB and Minimal medium


ABSTRACT: Gene Expression profiles of E.coli grown in LB and Minimal medium (DM) at OD 600 =1

ORGANISM(S): Escherichia coli

SUBMITTER: Paolo Boccazzi 

PROVIDER: E-GEOD-1981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data.

Boccazzi Paolo P   Zanzotto Andrea A   Szita Nicolas N   Bhattacharya Sanchita S   Jensen Klavs F KF   Sinskey Anthony J AJ  

Applied microbiology and biotechnology 20050901 4


Combining high-throughput growth physiology and global gene expression data analysis is of significant value for integrating metabolism and genomics. We compared global gene expression using 500 ng of total RNA from Escherichia coli cultures grown in rich or defined minimal media in a miniaturized 50-microl bioreactor. The microbioreactor was fabricated out of poly(dimethylsiloxane) (PDMS) and glass and equipped to provide on-line, optical measurements. cDNA labeling for microarray hybridization  ...[more]

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