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Thymine starvation and limitation in Escherichia coli thyA- cells

ABSTRACT: Transcriptional profiling of responses of E. coli thymine auxotrophs to i) growth under sub-optimal thymine concentration (TLM) and ii) lethal conditions under complete thymine starvation (TLD). Thymine limitation: Time series following shift to limiting thymine concentrations (15, 30, 45, 60 and 90 mins) - single replicate. Thymine starvation: Time series following shift to zero thymine medium (15, 30, 45, 60 and 90 mins) - 2 biological replicates at each time point.

ORGANISM(S): Escherichia coli

SUBMITTER: Dipen Sangurdekar

PROVIDER: E-GEOD-19879 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Thymineless death is associated with loss of essential genetic information from the replication origin.

Sangurdekar Dipen P DP Hamann Bree L BL Smirnov Dmitri D Srienc Friedrich F Hanawalt Philip C PC Khodursky Arkady B AB

Molecular microbiology 20100201 6

Thymine starvation results in a terminal cellular condition known as thymineless death (TLD), which is the basis of action for several common antibiotics and anticancer drugs. We characterized the onset and progression of TLD in Escherichia coli and found that DNA damage is the only salient property that distinguishes cells irreversibly senesced under thymine starvation from cells reversibly arrested by the nucleotide limitation. The damage is manifested as the relative loss of genetic material ...[more]

PMID: 20132444

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