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RNA-Seq of oral squamous cell carcinomas and matched normal tissues

ABSTRACT: RNA-Seq was applied to oral squamous cell carcinomas and matched normal oral tissue to measure gene expression patterns and identify examples of allelic imbalance. Oral squamous cell carcinomas (OSCC) and matched normal tissue from 3 patients.

ORGANISM(S): Homo sapiens

SUBMITTER: Brian Tuch

PROVIDER: E-GEOD-20116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tumor transcriptome sequencing reveals allelic expression imbalances associated with copy number alterations.

Tuch Brian B BB Laborde Rebecca R RR Xu Xing X Gu Jian J Chung Christina B CB Monighetti Cinna K CK Stanley Sarah J SJ Olsen Kerry D KD Kasperbauer Jan L JL Moore Eric J EJ Broomer Adam J AJ Tan Ruoying R Brzoska Pius M PM Muller Matthew W MW Siddiqui Asim S AS Asmann Yan W YW Sun Yongming Y Kuersten Scott S Barker Melissa A MA De La Vega Francisco M FM Smith David I DI

PloS one 20100219 2

Due to growing throughput and shrinking cost, massively parallel sequencing is rapidly becoming an attractive alternative to microarrays for the genome-wide study of gene expression and copy number alterations in primary tumors. The sequencing of transcripts (RNA-Seq) should offer several advantages over microarray-based methods, including the ability to detect somatic mutations and accurately measure allele-specific expression. To investigate these advantages we have applied a novel, strand-spe ...[more]

PMID: 20174472

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