Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Breast Cancer: Invasive Ductal Carcinoma

ABSTRACT: Invasive breast cancer was histopathologically confirmed on 47 samples obtained by core or incisional biopsy. Keywords: other

ORGANISM(S): Homo sapiens

SUBMITTER: Renato Puga

PROVIDER: E-GEOD-2048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression profiling of clinical stages II and III breast cancer.

Folgueira M A A K MA Brentani H H Katayama M L H ML Patrão D F C DF Carraro D M DM Mourão Netto M M Barbosa E M EM Caldeira J R F JR Abreu A P S AP Lyra E C EC Kaiano J H L JH Mota L D LD Campos A H J F M AH Maciel M S MS Dellamano M M Caballero O L S D OL Brentani M M MM

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 20060801 8

Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were di ...[more]

PMID: 16906285

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Project description:Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer where cells restricted to the ducts exhibit an atypical phenotype. Some DCIS lesions are believed to rapidly transit to invasive ductal carcinomas (IDCs), while others remain unchanged. Existing classification systems for DCIS fail to identify those lesions that transit to IDC. We studied gene expression patterns of 31 pure DCIS, 36 pure invasive cancers and 42 cases of mixed diagnosis (invasive cancer with an in situ component) using Agilent Whole Human Genome Oligo Microarrays 44k. Six normal breast tissue samples were also included as controls. qRT-PCR was used for validation. All DCIS and invasive samples could be classified into the intrinsic molecular subtypes defined for invasive breast cancer. Hierarchical clustering establishes that samples group by intrinsic subtype, and not by diagnosis. We observed heterogeneity in the transcriptomes among DCIS of high histological grade and identified a distinct subgroup containing seven of the 31 DCIS samples with gene expression characteristics more similar to advanced tumours. A set of genes independent of grade, ER-status and HER2-status was identified by logistic regression that univariately classified a sample as belonging to this distinct DCIS subgroup. qRT-PCR of single markers clearly separated this DCIS subgroup from the other DCIS, and contains samples from several histopathological and intrinsic molecular subtypes. The genes that differentiate between these two types of DCIS suggest several processes related to the re-organisation of the microenvironment. This raises interesting possibilities for identification of DCIS lesions both with and without invasive characteristics, which potentially could be used in clinical assessment of a woman's risk of progression, and lead to improved management that would avoid the current over- and under-treatment of patients. Breast cancer samples, 31 pure DCIS patients, 36 IDC patients, 42 mixed and 6 normal.

Dataset Information

Breast Cancer: Invasive Ductal Carcinoma

Publications

Gene expression profiling of clinical stages II and III breast cancer.

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