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Transcriptional profiling of Sarcophaga crassipalpis pupal diapause

ABSTRACT: Gene expression microarray exprement comparing differential expression between: 1) Early Diapause (ED), 2) Late Diapause (LD), 3) Non-Diapause (ND), 4) Hexane-induced diapause break (HEX). Four phenotypes (ED,ND,LD,HEX), four replicate pools of four individuals (four individuals in each replicate) in each phenotype, four competitve hybs comparing each phenotype to every other phenotype.

ORGANISM(S): Sarcophaga crassipalpis

SUBMITTER: Greg Ragland

PROVIDER: E-GEOD-20526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mechanisms of suspended animation are revealed by transcript profiling of diapause in the flesh fly.

Ragland Gregory J GJ Denlinger David L DL Hahn Daniel A DA

Proceedings of the National Academy of Sciences of the United States of America 20100728 33

Diapause is a widespread adaptation to seasonality across invertebrate taxa. It is critical for persistence in seasonal environments, synchronizing life histories with favorable, resource-rich conditions and mitigating exposure to harsh environments. Despite some promising recent progress, however, we still know very little about the molecular modifications underlying diapause. We used transcriptional profiling to identify key groups of genes and pathways differentially regulated during pupal di ...[more]

PMID: 20668242

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