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Intracellular Signaling Pathways in Nonviral Gene Delivery: Gene Expression Profiles in Transfected Cells

ABSTRACT: Analysis of gene expression changes due to nonviral gene delivery of DNA lipoplexes versus control in human HEK293T cells. Human HEK293T RNA was isolated from control, non-transfected cells (CTR) and transfected (GFP) samples for analysis on microarrays with three biological replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Jean-Jack Riethoven

PROVIDER: E-GEOD-20615 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of gene expression profiles in cells transfected with nonviral vectors.

Plautz Sarah A SA Boanca Gina G Riethoven Jean-Jack M JJ Pannier Angela K AK

Molecular therapy : the journal of the American Society of Gene Therapy 20110809 12

Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolat ...[more]

PMID: 21829178

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