Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Arabidopsis short day leaves overexpression miR319a

ABSTRACT: jaw-D plants grown in SD compared to columbia wt.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Javier Fernando Palatnik

PROVIDER: E-GEOD-2081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Specific effects of microRNAs on the plant transcriptome.

Schwab Rebecca R Palatnik Javier F JF Riester Markus M Schommer Carla C Schmid Markus M Weigel Detlef D

Developmental cell 20050401 4

Most plant microRNAs (miRNAs) have perfect or near-perfect complementarity with their targets. This is consistent with their primary mode of action being cleavage of target mRNAs, similar to that induced by perfectly complementary small interfering RNAs (siRNAs). However, there are natural targets with up to five mismatches. Furthermore, artificial siRNAs can have substantial effects on so-called off-targets, to which they have only limited complementarity. By analyzing the transcriptome of plan ...[more]

PMID: 15809034

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Project description:The Atss3 mutant and WT plants were arranged according to a Randomized Complete Block Design. The plants were planted in rows with seven rows in each flat; two plants of the same genotype/pot. Plants were grown under a SD photoperiod (8 h light/16 h dark) in a growth chamber as described. Eight randomly selected rows were harvested for each time point from different flats. Plant material was harvested at five time points in the diurnal cycle (1, 4, 8.5, 12, and 16 h; Time 0 is the beginning of the light period); harvesting was conducted under a green safety light. Each sample consisted of rosette leaves (leaves 5 to 8, staged following Bowmann (1994); photosynthetically active (Stessman et al., 2002)) from sixteen six-week-old plants. Leaf samples were frozen in liquid N2 immediately after harvest and stored at -80ï°C for RNA extraction. The experiment was done twice and independent randomizations for plant growth and harvest were used for the two replicates. Experiment Overall Design: The Atss3 mutant and WT plants were arranged according to a Randomized Complete Block Design. The plants were planted in rows with seven rows in each flat; two plants of the same genotype/pot. Plants were grown under a SD photoperiod (8 h light/16 h dark) in a growth chamber as described. Eight randomly selected rows were harvested for each time point from different flats. Plant material was harvested at five time points in the diurnal cycle (1, 4, 8.5, 12, and 16 h; Time 0 is the beginning of the light period); harvesting was conducted under a green safety light. Each sample consisted of rosette leaves (leaves 5 to 8, staged following Bowmann (1994); photosynthetically active (Stessman et al., 2002)) from sixteen six-week-old plants. Leaf samples were frozen in liquid N2 immediately after harvest and stored at -80ï°C for RNA extraction. The experiment was done twice and independent randomizations for plant growth and harvest were used for the two replicates.

Dataset Information

Transcription profiling of Arabidopsis short day leaves overexpression miR319a

Publications

Specific effects of microRNAs on the plant transcriptome.

Similar Datasets

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