Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome changes in soybean seeds resulting from RNAi suppression of seed storage proteins


ABSTRACT: We report the changes in transcriptome that results from RNAi suppression of 7S and 11S storage protein synthesis in soybean seeds, Proteomic results show that other proteins compensate for the storage protein shortfall. The transcriptome analysis show numerous changes result from suppressing storage proteins but compensating proteins are accumulated without parallel changes in the protein's transcription. Total RNA samples prepared from midmaturation wildtype and storage protein RNAi (SP-) soybeans were used to produce cDNA that was subjected to Illumina sequencing. The resulting sequences were assembled and analyzed to characterize the changes in transcriptome that results from suppressing storage protein synthesis/accumulation.

ORGANISM(S): Glycine max

SUBMITTER: Eliot Herman 

PROVIDER: E-GEOD-21116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome.

Schmidt Monica A MA   Barbazuk W Brad WB   Sandford Michael M   May Greg G   Song Zhihong Z   Zhou Wenxu W   Nikolau Basil J BJ   Herman Eliot M EM  

Plant physiology 20110310 1


The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to the wild type, maturing to similar size and weight, and in developmental ontogeny. The SP- seeds rebalance the proteome, maintaining wild-type levels of protein and storage triglyc  ...[more]

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