Unknown,Transcriptomics,Genomics,Proteomics

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Skeletal muscle of glucocorticoid deprived and treated mice by SAGE


ABSTRACT: Forty male C57BL6 mice 12-15 weeks old fed chow ad libitum. 30 mice underwent adrenalectomy (Adx) and 10 others a sham-Adx. Three or 24 h before tissue sampling, groups of 10 mice received an I.P. injection of cortisol (0.1 mg per mice), while the remaining Adx and intact mice received an I.P. injection of the vehicle (ethanol) 24 h prior to the sacrifice. All mice were killed within a period of a few hours. Gastrocnemius muscle were dissected and pooled together for each one of the four groups. Total RNA was isolated by Trizol. Keywords: other

ORGANISM(S): Mus musculus

SUBMITTER: Andre Boivin 

PROVIDER: E-GEOD-2122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Reproducibility, bioinformatic analysis and power of the SAGE method to evaluate changes in transcriptome.

Dinel S S   Bolduc C C   Belleau P P   Boivin A A   Yoshioka M M   Calvo E E   Piedboeuf B B   Snyder E E EE   Labrie F F   St-Amand J J  

Nucleic acids research 20050216 3


The serial analysis of gene expression (SAGE) method is used to study global gene expression in cells or tissues in various experimental conditions. However, its reproducibility has not yet been definitively assessed. In this study, we have evaluated the reproducibility of the SAGE method and identified the factors that affect it. The determination coefficient (R2 ) for the reproducibility of SAGE is 0.96. However, there are some factors that can affect the reproducibility of SAGE, such as the r  ...[more]

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